| Spectroscopic properties and photodynamic effects of new lipophilic porphyrin derivatives: efficacy, localisation and cell death pathways. | |
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MedLine Citation:
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PMID: 16495073 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Photodynamic therapy (PDT) and photodynamic diagnostics (PDD) of cancer are based on the use of non-toxic dyes (photosensitisers) in combination with harmless visible light. This paper reports physicochemical properties, cell uptake, localisation as well as photodynamic efficiency of two novel lipophilic porphyrin derivatives, suitable for use as PDT sensitisers. Both compounds are characterised by high quantum yield of singlet oxygen generation which was measured by time-resolved phosphorescence. Photodynamic in vitro studies were conducted on three cancer cell lines. Results of cell survival tests showed negligible dark cytotoxicity but high phototoxicity. The results also indicate that cell death is dependent on energy dose and time following light exposure. Using confocal laser scanning microscopy both compounds were found to localise in the cytoplasm around the nucleus of the tumour cells. The mode of cell death was evaluated based on the morphological changes after differential staining. In summary, good photostability, high quantum yield of singlet oxygen and biological effectiveness indicate that the examined lipophilic porphyrin derivatives offer quite interesting prospects of photodynamic therapy application. |
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Authors:
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Gabriela Kramer-Marek; Carlos Serpa; Agnieszka Szurko; Maria Widel; Aleksander Sochanik; Miroslaw Snietura; Piotr Kus; Rui M D Nunes; Luis G Arnaut; Alicja Ratuszna |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2006-02-21 |
Journal Detail:
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Title: Journal of photochemistry and photobiology. B, Biology Volume: 84 ISSN: 1011-1344 ISO Abbreviation: J. Photochem. Photobiol. B, Biol. Publication Date: 2006 Jul |
Date Detail:
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Created Date: 2006-06-13 Completed Date: 2006-10-04 Revised Date: 2007-07-23 |
Medline Journal Info:
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Nlm Unique ID: 8804966 Medline TA: J Photochem Photobiol B Country: Switzerland |
Other Details:
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Languages: eng Pagination: 1-14 Citation Subset: IM |
Affiliation:
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A.Chelkowski Institute of Physics, University of Silesia, Uniwersytecka 4, 40-007 Katowice, Poland. kramer_gabriela@yahoo.uk |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Cell Death
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drug effects*,
radiation effects Cell Line, Tumor Drug Screening Assays, Antitumor Humans Liposomes Molecular Structure Photochemistry Photochemotherapy Porphyrins / chemical synthesis, chemistry*, pharmacology* Signal Transduction / drug effects*, radiation effects Spectrum Analysis |
| Chemical | |
Reg. No./Substance:
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0/5-(4-hexadecyloxyphenyl)-10,15,20-tripyridylporphyrin; 0/5-(4-hydroxyphenyl)-10,15,20-tritotylporphyrin; 0/Liposomes; 0/Porphyrins |
| Comments/Corrections | |
Erratum In:
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J Photochem Photobiol B. 2006 Dec 1;85(3):228 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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