| Spectroscopic and photochemical characterization of the red-light sensitive photosensory module of Cph2 from Synechocystis PCC 6803. | |
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MedLine Citation:
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PMID: 21091956 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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Cyanobacterial phytochromes are a diverse family of light receptors controlling various biological functions including phototaxis. In addition to canonical bona fide phytochromes of the well characterized Cph1/plant-like clade, cyanobacteria also harbor phytochromes that absorb green, violet or blue light. The Synechocystis PCC 6803 Cph2 photoreceptor, a phototaxis inhibitor, is unconventional in bearing two distinct chromophore-binding GAF domains. Whereas the C-terminal GAF domain is most likely involved in blue-light perception, the first two domains correspond to a Cph1-like photosensory module lacking the PAS domain. Biochemical and spectroscopic studies show that this region switches between red (P(r) ) and far-red (P(fr) ) absorbing states. Unlike Cph1, the P(fr) state of Cph2 decays rapidly in darkness. Mutations close to the PCB chromophore further destabilize the P(fr) state without drastically affecting the spectroscopic features such as the quantum efficiency of P(r) →P(fr) conversion, fluorescence, or the Resonance-Raman signature of the chromophore. Overall, the PAS-less photosensory module of Cph2 resembles Cph1 including its mode of isomerisation, but the P(fr) state is unstable. |
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Authors:
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Katrin Anders; David von Stetten; Jo Mailliet; Stephan Kiontke; Vitaly A Sineshchekov; Peter Hildebrandt; Jon Hughes; Lars-Oliver Essen |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-11-23 |
Journal Detail:
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Title: Photochemistry and photobiology Volume: 87 ISSN: 1751-1097 ISO Abbreviation: Photochem. Photobiol. Publication Date: 2011 Jan-Feb |
Date Detail:
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Created Date: 2010-12-23 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0376425 Medline TA: Photochem Photobiol Country: United States |
Other Details:
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Languages: eng Pagination: 160-73 Citation Subset: IM |
Copyright Information:
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© 2010 The Authors. Photochemistry and Photobiology © 2010 The American Society of Photobiology. |
Affiliation:
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Department of Chemistry, Philipps University, Marburg, Germany. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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