| Spectroscopic determination of skin viability. A predictor of postmortem interval. | |
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MedLine Citation:
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PMID: 10641917 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We have demonstrated that skin viability decreases at a measurable rate following death in an animal model. The decreased skin viability was measured by fluorescein diacetate and ethidium bromide using fluorescence emission spectroscopy. There is significant decrease of the fluorescence intensity of the fluorescein diacetate assay between the 1-4 h, the 6-24 h, and the >40 h time points postmortem. For times between 6-24 h and >40 h postmortem the ethidium bromide assay showed consistent and significant increases in signal. The fluorescence measurements in this study showed that under the experimental conditions the time of death could be determined for <4, 6-24, and >40 hapotmotrem. The application of these assays in the field will require further study of the environmental factors. |
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Authors:
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A G Doukas; M Bamberg; R Gillies; R Evans; N Kollias |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of forensic sciences Volume: 45 ISSN: 0022-1198 ISO Abbreviation: J. Forensic Sci. Publication Date: 2000 Jan |
Date Detail:
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Created Date: 2000-02-25 Completed Date: 2000-02-25 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0375370 Medline TA: J Forensic Sci Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 36-41 Citation Subset: IM |
Affiliation:
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Wellman Laboratories of Photomedicine, Massachusetts General Hospital, Department of Dermatology, Harvard Medical School, Boston 02114, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Fluorescein Male Postmortem Changes* Skin / pathology* Spectrometry, Fluorescence / methods* Swine Time Factors |
| Chemical | |
Reg. No./Substance:
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2321-07-5/Fluorescein |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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