Document Detail


Spectroscopic determination of skin viability. A predictor of postmortem interval.
MedLine Citation:
PMID:  10641917     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have demonstrated that skin viability decreases at a measurable rate following death in an animal model. The decreased skin viability was measured by fluorescein diacetate and ethidium bromide using fluorescence emission spectroscopy. There is significant decrease of the fluorescence intensity of the fluorescein diacetate assay between the 1-4 h, the 6-24 h, and the >40 h time points postmortem. For times between 6-24 h and >40 h postmortem the ethidium bromide assay showed consistent and significant increases in signal. The fluorescence measurements in this study showed that under the experimental conditions the time of death could be determined for <4, 6-24, and >40 hapotmotrem. The application of these assays in the field will require further study of the environmental factors.
Authors:
A G Doukas; M Bamberg; R Gillies; R Evans; N Kollias
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of forensic sciences     Volume:  45     ISSN:  0022-1198     ISO Abbreviation:  J. Forensic Sci.     Publication Date:  2000 Jan 
Date Detail:
Created Date:  2000-02-25     Completed Date:  2000-02-25     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0375370     Medline TA:  J Forensic Sci     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  36-41     Citation Subset:  IM    
Affiliation:
Wellman Laboratories of Photomedicine, Massachusetts General Hospital, Department of Dermatology, Harvard Medical School, Boston 02114, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Fluorescein
Male
Postmortem Changes*
Skin / pathology*
Spectrometry, Fluorescence / methods*
Swine
Time Factors
Chemical
Reg. No./Substance:
2321-07-5/Fluorescein

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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