| Spectroscopic characterization of microorganisms by Fourier transform infrared microspectroscopy. | |
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MedLine Citation:
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PMID: 15700299 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Spectroscopic fingerprints of bacteria were investigated by Fourier transform infrared (FTIR) microspectroscopy for the elucidation of chemical composition and structural information during growth. Good differentiation of six microorganisms was achieved down to the strain level. The inherent compositional and structural differences of cell envelopes and cytoplasm were investigated and utilized to obtain more detailed analysis of the spectroscopic features. Bands or regions of key functional groups were also identified in the original spectra. Microspectroscopic monitoring of bacterial growth demonstrated that FTIR spectroscopy cannot only provide molecular fingerprints of the cell envelope, but also compositional and metabolic information of the cytoplasm under different physiological conditions. This approach could be an effective alternative to traditional nutritional and biochemical methods to monitor and assess the effects of inhibitors and other environmental factors on microbial cell growth. |
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Authors:
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Chenxu Yu; Joseph Irudayaraj |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Biopolymers Volume: 77 ISSN: 0006-3525 ISO Abbreviation: Biopolymers Publication Date: 2005 Apr |
Date Detail:
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Created Date: 2005-03-23 Completed Date: 2005-05-12 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0372525 Medline TA: Biopolymers Country: United States |
Other Details:
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Languages: eng Pagination: 368-77 Citation Subset: IM |
Copyright Information:
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Copyright 2005 Wiley Periodicals, Inc. |
Affiliation:
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Department of Agricultural and Biological Engineering, Pennsylvania State University, State College, PA 16802, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Escherichia coli
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chemistry* Molecular Conformation Salmonella enteritidis / chemistry* Salmonella typhi / chemistry* Shigella boydii / chemistry* Spectrophotometry, Infrared Spectroscopy, Fourier Transform Infrared / methods Yersinia enterocolitica / chemistry* |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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