Document Detail


Spectroscopic characterization of microorganisms by Fourier transform infrared microspectroscopy.
MedLine Citation:
PMID:  15700299     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Spectroscopic fingerprints of bacteria were investigated by Fourier transform infrared (FTIR) microspectroscopy for the elucidation of chemical composition and structural information during growth. Good differentiation of six microorganisms was achieved down to the strain level. The inherent compositional and structural differences of cell envelopes and cytoplasm were investigated and utilized to obtain more detailed analysis of the spectroscopic features. Bands or regions of key functional groups were also identified in the original spectra. Microspectroscopic monitoring of bacterial growth demonstrated that FTIR spectroscopy cannot only provide molecular fingerprints of the cell envelope, but also compositional and metabolic information of the cytoplasm under different physiological conditions. This approach could be an effective alternative to traditional nutritional and biochemical methods to monitor and assess the effects of inhibitors and other environmental factors on microbial cell growth.
Authors:
Chenxu Yu; Joseph Irudayaraj
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biopolymers     Volume:  77     ISSN:  0006-3525     ISO Abbreviation:  Biopolymers     Publication Date:  2005 Apr 
Date Detail:
Created Date:  2005-03-23     Completed Date:  2005-05-12     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372525     Medline TA:  Biopolymers     Country:  United States    
Other Details:
Languages:  eng     Pagination:  368-77     Citation Subset:  IM    
Copyright Information:
Copyright 2005 Wiley Periodicals, Inc.
Affiliation:
Department of Agricultural and Biological Engineering, Pennsylvania State University, State College, PA 16802, USA.
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MeSH Terms
Descriptor/Qualifier:
Escherichia coli / chemistry*
Molecular Conformation
Salmonella enteritidis / chemistry*
Salmonella typhi / chemistry*
Shigella boydii / chemistry*
Spectrophotometry, Infrared
Spectroscopy, Fourier Transform Infrared / methods
Yersinia enterocolitica / chemistry*

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