| Spectroscopic studies on the binding of cobalt(II) 1,10-phenanthroline complex to bovine serum albumin. | |
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MedLine Citation:
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PMID: 19727573 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The binding interaction of the cobalt(II) 1,10-phenanthroline complex (Co(phen) (3) (2+) , phen = 1,10-phenanthroline) with bovine serum albumin (BSA) was investigated by fluorescence spectroscopy combined with UV-Vis absorption and circular dichroism measurements under simulative physiological conditions. The experiment results showed that the fluorescence intensity of BSA was dramatically decreased owing to the formation of Co(phen) (3) (2+) -BSA complex. The corresponding association constants (K (a)) between Co(phen) (3) (2+) and BSA at four different temperatures were calculated according to the modified Stern-Volmer equation. The enthalpy change (DeltaH degrees ) and entropy change (DeltaS degrees ) were calculated to be -2.73 kJ mol(-1) and 82.27 J mol(-1) K(-1), respectively, which suggested that electrostatic interaction and hydrophobic force played major roles in stabilizing the Co(phen) (3) (2+) -BSA complex. Site marker competitive experiments indicated that the binding of Co(phen) (3) (2+) to BSA primarily took place in site I of BSA. A value of 4.11 nm for the average distance r between Co(phen) (3) (2+) (acceptor) and tryptophan residues of BSA (donor) was derived from Förster's energy transfer theory. The conformational investigation showed that the presence of Co(phen) (3) (2+) resulted in the change of BSA secondary structure and induced the slight unfolding of the polypeptides of protein, which confirmed the microenvironment and conformational changes of BSA molecules. |
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Authors:
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Ye-Zhong Zhang; Hua-Rong Li; Jie Dai; Wen-Juan Chen; Jing Zhang; Yi Liu |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-09-02 |
Journal Detail:
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Title: Biological trace element research Volume: 135 ISSN: 1559-0720 ISO Abbreviation: Biol Trace Elem Res Publication Date: 2010 Jun |
Date Detail:
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Created Date: 2010-05-03 Completed Date: 2010-08-02 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7911509 Medline TA: Biol Trace Elem Res Country: United States |
Other Details:
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Languages: eng Pagination: 136-52 Citation Subset: IM |
Affiliation:
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College of Chemistry and Environmental Engineering, Yangtze University, Jingzhou, Hubei 434023, People's Republic of China. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Circular Dichroism Cobalt / metabolism* Organometallic Compounds / metabolism* Phenanthrolines / metabolism* Serum Albumin, Bovine / chemistry* Spectrometry, Fluorescence Spectrophotometry, Ultraviolet Thermodynamics |
| Chemical | |
Reg. No./Substance:
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0/Organometallic Compounds; 0/Phenanthrolines; 0/Serum Albumin, Bovine; 0/triphenanthrolinecobalt(II); 66-71-7/1,10-phenanthroline; 7440-48-4/Cobalt |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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