| Spectrophotometric monitoring of lipoxygenase and cyclooxygenase pathway activity using ionophore stimulated whole blood. | |
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MedLine Citation:
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PMID: 3120516 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We have employed clotting human blood stimulated with ionophore to develop a system for measuring cyclooxygenase, 5-lipoxygenase, and 12-lipoxygenase pathway products released into the serum fraction. In a single chromatographic run, 5-HETE, 12-HETE, 12-OH-heptadecatrienoic acid (HHT), LTB4, 20-OH-LTB4, and 20-COOH-LTB4 are quantitated by UV monitoring after separation by HPLC. The kinetics of product formation/release of all fatty acid products into the serum show an apparent lag of approximately 2 min, after which time the amounts of HHT, 5-HETE, and LTB4, respectively, plateau at 10 min while 12-HETE increases over a 60 min period. The system is responsive to standard cyclooxygenase and lipoxygenase inhibitors, and is of value of evaluating prospective blockers of AA metabolism in a whole blood setting. |
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Authors:
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F J Sweeney; J D Eskra; M J Ernest; T J Carty |
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Publication Detail:
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Type: In Vitro; Journal Article |
Journal Detail:
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Title: Agents and actions Volume: 21 ISSN: 0065-4299 ISO Abbreviation: Agents Actions Publication Date: 1987 Aug |
Date Detail:
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Created Date: 1988-01-19 Completed Date: 1988-01-19 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0213341 Medline TA: Agents Actions Country: SWITZERLAND |
Other Details:
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Languages: eng Pagination: 393-6 Citation Subset: IM |
Affiliation:
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Department of Immunology and Infectious Disease, Pfizer Central Research, Groton, CT 06340. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Calcimycin
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pharmacology Chromatography, High Pressure Liquid Fatty Acids, Unsaturated / blood Humans Lipoxygenase / blood* Prostaglandin-Endoperoxide Synthases / blood* Spectrophotometry, Ultraviolet |
| Chemical | |
Reg. No./Substance:
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0/Fatty Acids, Unsaturated; 52665-69-7/Calcimycin; EC 1.13.11.12/Lipoxygenase; EC 1.14.99.1/Prostaglandin-Endoperoxide Synthases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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