Document Detail

Spectrophotometric determination of protein concentration.
MedLine Citation:
PMID:  18429266     Owner:  NLM     Status:  MEDLINE    
The concentration of a purified protein in solution is most conveniently and accurately measured using absorbance spectroscopy. The absorbance, A, is a linear function of the molar concentration, C, according to the Beer-Lambert law: A = epsilon x l x c, where e is the molar absorption coefficient and l is the cell path length. This unit provides protocols for calculation of epsilon for a folded or unfolded protein, making use of the average epsilon values for the three contributing chromophores in proteins (the side chains of Trp, Tyr, and Cys). A basic protocol describes how to measure the concentration of a protein using the calculated epsilon and the Beer-Lambert law. A sensitive method is provided for measuring the concentration of proteins that contain few if any tryptophan or tyrosine residues, and a simple method is provided for estimating total protein concentration in crude extracts.
Gerald R Grimsley; C Nick Pace
Related Documents :
14716016 - Reflectins: the unusual proteins of squid reflective tissues.
18092766 - Photoreactions of tyr8- and gln50-mutated bluf domains of the pixd protein of thermosyn...
25384616 - The effect of propofol on the expression of rabbit ischemia reperfusion injury-related ...
16939366 - Structure and photoreaction of photoactive yellow protein, a structural prototype of th...
24530066 - The initiation of clathrin-mediated endocytosis is mechanistically highly flexible.
15939016 - The alpha helix dipole: screened out?
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Current protocols in protein science / editorial board, John E. Coligan ... [et al.]     Volume:  Chapter 3     ISSN:  1934-3663     ISO Abbreviation:  -     Publication Date:  2004 Nov 
Date Detail:
Created Date:  2008-04-22     Completed Date:  2008-08-08     Revised Date:  2008-12-15    
Medline Journal Info:
Nlm Unique ID:  101287868     Medline TA:  Curr Protoc Protein Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  Unit 3.1     Citation Subset:  IM    
The Texas A&M University System Health Science Center, College Station, Texas, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Protein Folding
Proteins / analysis*
Spectrophotometry, Ultraviolet / methods*
Reg. No./Substance:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Proteomic analysis using 2-D liquid separations of intact proteins from whole-cell lysates.
Next Document:  Subcellular fractionation of tissue culture cells.