| Spectrophotometric assay for superoxide dismutase based on tetrazolium salt 3'--1--(phenylamino)-carbonyl--3, 4-tetrazolium]-bis(4-methoxy-6-nitro)benzenesulfonic acid hydrate reduction by xanthine-xanthine oxidase. | |
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MedLine Citation:
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PMID: 9299017 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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XTT (3'-{1-[(phenylamino)-carbonyl]-3, 4-tetrazolium}bis(4-methoxy-6-nitro)benzenesulfonic acid hydrate) was reduced to a water-soluble product with an absorbance maximum at about 470 nm by superoxide anion generated by xanthine-xanthine oxidase (XO). The rate of XTT reduction was linearly related to XO activity and the reduction was inhibited by superoxide dismutase (SOD). A perfect inhibition of the reduction of XTT by SOD was achieved, suggesting that XTT does not interact with XO. The present XTT-based assay had a higher sensitivity than a conventional nitroblue tetrazolium-based assay by a factor of 2.5 at pH 10.2. This method was applicable to the SOD assay in the pH range 8.0-10.2. |
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Authors:
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H Ukeda; S Maeda; T Ishii; M Sawamura |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Analytical biochemistry Volume: 251 ISSN: 0003-2697 ISO Abbreviation: Anal. Biochem. Publication Date: 1997 Sep |
Date Detail:
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Created Date: 1997-10-29 Completed Date: 1997-10-29 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0370535 Medline TA: Anal Biochem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 206-9 Citation Subset: IM |
Copyright Information:
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Copyright 1997Academic Press |
Affiliation:
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Faculty of Agriculture, Kochi University, Monobe B-200, Nankoku, 783, Japan. hukeda@cc.kochi-u.ac.jp |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cattle Hydrogen-Ion Concentration Indicators and Reagents / metabolism* Oxidation-Reduction Superoxide Dismutase / analysis* Superoxides / metabolism Tetrazolium Salts / metabolism* Xanthine Oxidase / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Indicators and Reagents; 0/Tetrazolium Salts; 11062-77-4/Superoxides; 117038-70-7/2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-((phenylamino)carbonyl)-2H-tetrazolium hydroxide; EC 1.15.1.1/Superoxide Dismutase; EC 1.17.3.2/Xanthine Oxidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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