Document Detail


Spectrophotometric assay for superoxide dismutase based on tetrazolium salt 3'--1--(phenylamino)-carbonyl--3, 4-tetrazolium]-bis(4-methoxy-6-nitro)benzenesulfonic acid hydrate reduction by xanthine-xanthine oxidase.
MedLine Citation:
PMID:  9299017     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
XTT (3'-{1-[(phenylamino)-carbonyl]-3, 4-tetrazolium}bis(4-methoxy-6-nitro)benzenesulfonic acid hydrate) was reduced to a water-soluble product with an absorbance maximum at about 470 nm by superoxide anion generated by xanthine-xanthine oxidase (XO). The rate of XTT reduction was linearly related to XO activity and the reduction was inhibited by superoxide dismutase (SOD). A perfect inhibition of the reduction of XTT by SOD was achieved, suggesting that XTT does not interact with XO. The present XTT-based assay had a higher sensitivity than a conventional nitroblue tetrazolium-based assay by a factor of 2.5 at pH 10.2. This method was applicable to the SOD assay in the pH range 8.0-10.2.
Authors:
H Ukeda; S Maeda; T Ishii; M Sawamura
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Analytical biochemistry     Volume:  251     ISSN:  0003-2697     ISO Abbreviation:  Anal. Biochem.     Publication Date:  1997 Sep 
Date Detail:
Created Date:  1997-10-29     Completed Date:  1997-10-29     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0370535     Medline TA:  Anal Biochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  206-9     Citation Subset:  IM    
Copyright Information:
Copyright 1997Academic Press
Affiliation:
Faculty of Agriculture, Kochi University, Monobe B-200, Nankoku, 783, Japan. hukeda@cc.kochi-u.ac.jp
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MeSH Terms
Descriptor/Qualifier:
Animals
Cattle
Hydrogen-Ion Concentration
Indicators and Reagents / metabolism*
Oxidation-Reduction
Superoxide Dismutase / analysis*
Superoxides / metabolism
Tetrazolium Salts / metabolism*
Xanthine Oxidase / metabolism*
Chemical
Reg. No./Substance:
0/Indicators and Reagents; 0/Tetrazolium Salts; 11062-77-4/Superoxides; 117038-70-7/2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-((phenylamino)carbonyl)-2H-tetrazolium hydroxide; EC 1.15.1.1/Superoxide Dismutase; EC 1.17.3.2/Xanthine Oxidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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