| Spectrophotometric absorbance of follicular fluid: a predictor of oocyte fertilizing capability. | |
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MedLine Citation:
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PMID: 3342896 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Follicular fluid volume, scoring of the oocyte-cumulus-corona-complex (OCCC), and spectrophotometric absorbance of the follicular fluid were separately compared between a group of fertilized (n = 53) and unfertilized oocytes (n = 35). Scoring of the OCCC and follicular fluid volumes was not found to be statistically different between the two groups. Spectrophotometric analysis of the follicular fluid in the visible spectrum demonstrated two peaks of maximum absorbance at 415 nm and 455 nm. The group of oocytes that fertilized was associated with follicular fluids that had significantly higher absorbances at 415 nm and 455 nm. In conclusion, follicular fluid volume and scoring of the OCCC were poor predictors of fertilizing capability; however, spectrophotometric absorbance, particularly at 455 nm, was positively correlated with oocyte fertilization. |
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Authors:
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S R Bayer; D R Armant; A M Dlugi; M M Seibel |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Fertility and sterility Volume: 49 ISSN: 0015-0282 ISO Abbreviation: Fertil. Steril. Publication Date: 1988 Mar |
Date Detail:
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Created Date: 1988-03-29 Completed Date: 1988-03-29 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 0372772 Medline TA: Fertil Steril Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 442-6 Citation Subset: IM |
Affiliation:
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Dana Biomedical Research Laboratories, Beth Israel Hospital, Harvard Medical School, Boston, Massachusetts 02115. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Female Fertilization in Vitro* Humans Oocytes / anatomy & histology, physiology* Ovarian Follicle / analysis* Prospective Studies Spectrophotometry* |
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