| Spectrofluorometric determination of labetolol in pharmaceutical preparations and spiked human urine through the formation of coumarin derivative. | |
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MedLine Citation:
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PMID: 12408909 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A simple, sensitive and specific spectrofluorimetric method has been developed for the determination of labetalol (LBT). The method is based on the reaction between LBT and ethylacetoacetate in the presence of sulphuric acid to give yellow fluorescent product with excitation wavelength of 312 nm and emission wavelength of 432 nm. The reaction conditions were studied and optimized. The fluorescence intensity-concentration plot is rectilinear over the range 1-15 microgram/ml with minimum detectability limit of 0.8 microgram/ml (2.16 x 10(-6) M). The proposed method was successfully applied to commercial tablets containing LBT, the percentage recoveries agreed well with those obtained using the official methods. Hydrochlorothiazide, which is frequently co-formulated with LBT did not interfere with the assay. The method was further extended to the in-vitro determination of LBT in spiked human urine samples. The percentage recovery was 101.50+/-6.18 (n=6). A proposal of the reaction pathway was postulated. |
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Authors:
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F Belal; S Al-Shaboury; A S Al-Tamrah |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of pharmaceutical and biomedical analysis Volume: 30 ISSN: 0731-7085 ISO Abbreviation: J Pharm Biomed Anal Publication Date: 2002 Nov |
Date Detail:
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Created Date: 2002-10-31 Completed Date: 2003-04-22 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 8309336 Medline TA: J Pharm Biomed Anal Country: England |
Other Details:
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Languages: eng Pagination: 1191-6 Citation Subset: IM |
Affiliation:
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Department of Pharmaceutical Chemistry, College of Pharmacy, King Saud University, P.O. Box 2457, 11451, Riyadh, Saudi Arabia. ffbelal@yahoo.com |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Coumarins / analysis*, chemistry Humans Labetalol / analysis, chemistry, urine* Male Pharmaceutical Preparations Spectrometry, Fluorescence / methods |
| Chemical | |
Reg. No./Substance:
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0/Coumarins; 0/Pharmaceutical Preparations; 36894-69-6/Labetalol; 91-64-5/coumarin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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