Document Detail


Spectrofluorimetric techniques in the study of the interaction between fluorescent dyes and proteins or nuclear acids.
MedLine Citation:
PMID:  749334     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
After a review of the applications of spectrofluorimetric techniques to the study of ligand--biological substrate interactions, mention is made of the main fluorescent dyes used for the demonstration of structural characteristics and conformational alterations in proteins, nucleic acids, etc. The possibilities of using new compounds recommended in the literature (fluorescamine, fluoropa, a.o.) for the identification and study of proteins are analysed and original investigations with newly synthesized fluorescent compounds (anthraquinone dyes, anhydrides of peridicarboxylic acids, Tb3+ and Sm3+ complexes of the pyridine -2,6-dicarboxylic acid) are presented.
Authors:
C N Zaharia; C Tărăbăşanu-Mihăilă
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Virologie     Volume:  29     ISSN:  0377-8177     ISO Abbreviation:  Virologie     Publication Date:    1978 Oct-Dec
Date Detail:
Created Date:  1979-06-11     Completed Date:  1979-06-11     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  7605765     Medline TA:  Virologie     Country:  ROMANIA    
Other Details:
Languages:  eng     Pagination:  283-91     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Chemical Phenomena
Chemistry
Fluorescence
Fluorescent Dyes* / metabolism
Nucleic Acids* / metabolism
Protein Conformation
Proteins* / metabolism
Spectrometry, Fluorescence*
Chemical
Reg. No./Substance:
0/Fluorescent Dyes; 0/Nucleic Acids; 0/Proteins

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