Document Detail

Spectrofluorimetric determination of urinary p-aminobenzoic and p-aminosalicylic acids in the BT-PABA/PAS test of pancreatic function.
MedLine Citation:
PMID:  1769111     Owner:  NLM     Status:  MEDLINE    
Spectrofluorimetry was investigated as an alternative to HPLC for determining p-aminobenzoic acid and p-aminosalicylic acid in the N-benzoyl-L-tyrosyl-p-aminobenzoic acid/p-aminosalicylic acid test of pancreatic exocrine function. Urine specimens were hydrolysed for 30 min in 4 M NaOH at 100 degrees C. The fluorescence of p-aminobenzoic acid was measured in dimethyl sulphoxide solution (lambda ex = 300 nm, lambda em = 340 nm) and that of p-aminosalicylic acid in sodium acetate buffer, pH 4.0 (lambda ex = 297 nm, lambda em = 394 nm). The linear range was 0.038-8 mM for p-aminobenzoic acid and 0.051-12 mM for p-aminosalicylic acid, within-batch precision was 2.2% and 5.5%, respectively, and the entire analysis could be completed within 40 min. Although not eliminated, drug interference was greatly reduced in comparison with colorimetry. In 23 consecutive pancreatic function tests there was an excellent correlation between the p-aminobenzoic acid/p-aminosalicylic acid excretion index obtained by fluorimetry and the results from HPLC analysis (y = 0.914x + 0.070, r = 0.987, p less than 0.001). The method is simple, cost-effective and may be particularly valuable in developing countries having a high incidence of chronic pancreatitis.
P W Pemberton; P Gagjee; C Chaloner; J M Braganza; R W Lobley
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Clinica chimica acta; international journal of clinical chemistry     Volume:  199     ISSN:  0009-8981     ISO Abbreviation:  Clin. Chim. Acta     Publication Date:  1991 Jul 
Date Detail:
Created Date:  1992-02-27     Completed Date:  1992-02-27     Revised Date:  2010-08-25    
Medline Journal Info:
Nlm Unique ID:  1302422     Medline TA:  Clin Chim Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  253-62     Citation Subset:  IM    
University Department of Gastroenterology, Royal Infirmary, Manchester, England.
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MeSH Terms
4-Aminobenzoic Acid / analogs & derivatives*,  chemistry,  metabolism,  urine*
Aminosalicylic Acid / chemistry,  metabolism,  urine*
Chromatography, High Pressure Liquid
Dimethyl Sulfoxide
Pancreatic Function Tests / methods*
Pancreatitis / diagnosis
Spectrometry, Fluorescence*
Grant Support
//Wellcome Trust
Reg. No./Substance:
150-13-0/4-Aminobenzoic Acid; 37106-97-1/bentiromide; 65-49-6/Aminosalicylic Acid; 67-68-5/Dimethyl Sulfoxide

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