Document Detail


Spectrally-resolved fluorescence cross sections of aerosolized biological live agents and simulants using five excitation wavelengths in a BSL-3 laboratory.
MedLine Citation:
PMID:  24718194     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
A system for measuring spectrally-resolved fluorescence cross sections of single bioaerosol particles has been developed and employed in a biological safety level 3 (BSL-3) facility at Edgewood Chemical and Biological Center (ECBC). It is used to aerosolize the slurry or solution of live agents and surrogates into dried micron-size particles, and to measure the fluorescence spectra and sizes of the particles one at a time. Spectrally-resolved fluorescence cross sections were measured for (1) bacterial spores: Bacillus anthracis Ames (BaA), B. atrophaeus var. globigii (BG) (formerly known as Bacillus globigii), B. thuringiensis israelensis (Bti), B. thuringiensis kurstaki (Btk), B. anthracis Sterne (BaS); (2) vegetative bacteria: Escherichia coli (E. coli), Pantoea agglomerans (Eh) (formerly known as Erwinia herbicola), Yersinia rohdei (Yr), Yersinia pestis CO92 (Yp); and (3) virus preparations: Venezuelan equine encephalitis TC83 (VEE) and the bacteriophage MS2. The excitation wavelengths were 266 nm, 273 nm, 280 nm, 365 nm and 405 nm.
Authors:
Yong-Le Pan; Steven C Hill; Joshua L Santarpia; Kelly Brinkley; Todd Sickler; Mark Coleman; Chatt Williamson; Kris Gurton; Melvin Felton; Ronald G Pinnick; Neal Baker; Jonathan Eshbaugh; Jerry Hahn; Emily Smith; Ben Alvarez; Amber Prugh; Warren Gardner
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Optics express     Volume:  22     ISSN:  1094-4087     ISO Abbreviation:  Opt Express     Publication Date:  2014 Apr 
Date Detail:
Created Date:  2014-04-10     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101137103     Medline TA:  Opt Express     Country:  United States    
Other Details:
Languages:  eng     Pagination:  8165-89     Citation Subset:  IM    
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