Document Detail


Spectral imaging fluorescence microscopy.
MedLine Citation:
PMID:  12296819     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The spectral resolution of fluorescence microscope images in living cells is achieved by using a confocal laser scanning microscope equipped with grating optics. This capability of temporal and spectral resolution is especially useful for detecting spectral changes of a fluorescent dye; for example, those associated with fluorescence resonance energy transfer (FRET). Using the spectral imaging fluorescence microscope system, it is also possible to resolve emitted signals from fluorescent dyes that have spectra largely overlapping with each other, such as fluorescein isothiocyanate (FITC) and green fluorescent protein (GFP).
Authors:
Tokuko Haraguchi; Takeshi Shimi; Takako Koujin; Noriyo Hashiguchi; Yasushi Hiraoka
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Genes to cells : devoted to molecular & cellular mechanisms     Volume:  7     ISSN:  1356-9597     ISO Abbreviation:  Genes Cells     Publication Date:  2002 Sep 
Date Detail:
Created Date:  2002-09-25     Completed Date:  2003-04-18     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9607379     Medline TA:  Genes Cells     Country:  England    
Other Details:
Languages:  eng     Pagination:  881-7     Citation Subset:  IM    
Affiliation:
CREST Research Project, Kansai Advanced Research Center, Communications Research Laboratory, 588-2 Iwaoka, Iwaoka-cho, Nishi-ku, Kobe 651-2492, Japan.
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MeSH Terms
Descriptor/Qualifier:
Fluorescein-5-isothiocyanate / metabolism
Fluorescence Resonance Energy Transfer
Fluorescent Dyes / metabolism*
Green Fluorescent Proteins
Hela Cells
Humans
Luminescent Proteins / metabolism
Microscopy, Confocal
Microscopy, Fluorescence / methods*
Recombinant Fusion Proteins / metabolism
Chemical
Reg. No./Substance:
0/Fluorescent Dyes; 0/Luminescent Proteins; 0/Recombinant Fusion Proteins; 147336-22-9/Green Fluorescent Proteins; 3326-32-7/Fluorescein-5-isothiocyanate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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