Document Detail

Spectral diffusion induced by proton dynamics in pigment-protein complexes.
MedLine Citation:
PMID:  19053184     Owner:  NLM     Status:  MEDLINE    
The fluorescence emission of individual photosystem I complexes from Synechocystis PCC 6803 in protonated and deuterated buffer shows zero-phonon lines as well as broad intensity distributions. The number and the line width of the zero phonon lines depend strongly on the solvent (H(2)O/D(2)O). The spectral diffusion rate of the whole fluorescence emission from photosystem I is significantly reduced upon deuteration of the solvent. This leads to a substantial increase of well-resolved zero-phonon lines. Since the chlorophyll a chromophores lack exchangeable protons, these observed changes in the spectral diffusion have to be assigned to exchangeable protons at the amino acids and structural water molecules in the chromophore binding pocket.
Marc Brecht; Hauke Studier; Volker Radics; Jana B Nieder; Robert Bittl
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of the American Chemical Society     Volume:  130     ISSN:  1520-5126     ISO Abbreviation:  J. Am. Chem. Soc.     Publication Date:  2008 Dec 
Date Detail:
Created Date:  2008-12-18     Completed Date:  2009-08-05     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7503056     Medline TA:  J Am Chem Soc     Country:  United States    
Other Details:
Languages:  eng     Pagination:  17487-93     Citation Subset:  IM    
Fachbereich Physik, Freie Universitat Berlin, Arnimalle 14, 14195 Berlin, Germany.
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MeSH Terms
Amino Acids / chemistry
Chlorophyll / chemistry
Deuterium / chemistry
Hydrogen Bonding
Models, Chemical
Proteins / chemistry
Solvents / chemistry
Spectrometry, Fluorescence / methods
Spectrophotometry / methods
Synechocystis / metabolism*
Water / chemistry
Reg. No./Substance:
0/Amino Acids; 0/Proteins; 0/Protons; 0/Solvents; 1406-65-1/Chlorophyll; 479-61-8/chlorophyll a; 7732-18-5/Water; 7782-39-0/Deuterium

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