Document Detail

Speckle variance detection of microvasculature using swept-source optical coherence tomography.
MedLine Citation:
PMID:  18594688     Owner:  NLM     Status:  MEDLINE    
We report on imaging of microcirculation by calculating the speckle variance of optical coherence tomography (OCT) structural images acquired using a Fourier domain mode-locked swept-wavelength laser. The algorithm calculates interframe speckle variance in two-dimensional and three-dimensional OCT data sets and shows little dependence to the Doppler angle ranging from 75 degrees to 90 degrees . We demonstrate in vivo detection of blood flow in vessels as small as 25 microm in diameter in a dorsal skinfold window chamber model with direct comparison with intravital fluorescence confocal microscopy. This technique can visualize vessel-size-dependent vascular shutdown and transient vascular occlusion during Visudyne photodynamic therapy and may provide opportunities for studying therapeutic effects of antivascular treatments without on exogenous contrast agent.
Adrian Mariampillai; Beau A Standish; Eduardo H Moriyama; Mamta Khurana; Nigel R Munce; Michael K Leung; James Jiang; Alex Cable; Brian C Wilson; I Alex Vitkin; Victor X D Yang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Optics letters     Volume:  33     ISSN:  0146-9592     ISO Abbreviation:  Opt Lett     Publication Date:  2008 Jul 
Date Detail:
Created Date:  2008-07-02     Completed Date:  2008-09-16     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7708433     Medline TA:  Opt Lett     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1530-2     Citation Subset:  IM    
Department of Medical Biophysics, University of Toronto, Toronto, M5G 2M9, Canada.
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MeSH Terms
Image Enhancement / methods*
Image Interpretation, Computer-Assisted / methods*
Mice, Nude
Microcirculation / cytology*
Microscopy, Confocal / methods*
Microscopy, Fluorescence / methods*
Reproducibility of Results
Sensitivity and Specificity
Tomography, Optical Coherence / methods*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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