Document Detail

Specificity of subnormal deltaPO2 for retinal neovascularization in experimental retinopathy of prematurity.
MedLine Citation:
PMID:  12882806     Owner:  NLM     Status:  MEDLINE    
PURPOSE: To test the hypothesis that in an experimental model of retinopathy of prematurity (ROP) rat pups that are at risk for but do not have retinal neovascularization (NV) exhibit a normal oxygenation response to a hyperoxic inhalation challenge. METHODS: Newborn Sprague-Dawley rats were raised under two varied oxygen conditions (50/10 or 40/15, indicating percent of oxygen in the air on alternate days) for 14 days and then allowed to recover in room air. Functional magnetic resonance imaging was used to determine the retinal oxygenation response (increase in partial oxygen pressure in the vitreous over the room air value, or deltaPO(2), in mm Hg) to hyperoxic inhalation challenge. Adenosine diphosphatase (ADPase)-stained retinas were analyzed to determine the NV incidence and severity. RESULTS: On postnatal day (P)20, the 40/15 procedure produced significantly (P < 0.05) lower incidence of NV than the 50/10 protocol (8% vs. 99%, respectively). Retinal deltaPO(2) during carbogen breathing of the 40/15 animals that did not have evidence of NV was not different (P > 0.05) from that of normal age-matched animals; later time points were not examined. At P26 and P34, in 50/10 rats that no longer had NV, retinal deltaPO(2)s during carbogen breathing were significantly (P < 0.05) lower than that of age-matched control pups. At P34 in control rats, but not in 50/10 rats, deltaPO(2) was 61% greater (P < 0.05) during carbogen breathing than during oxygen breathing. CONCLUSIONS: The results from 40/15 experiments, together with the authors' previous data in 50/10 rats, which documented subnormal retinal deltaPO(2) before and during NV, provides additional support that subnormal retinal oxygenation to an inhalation challenge is an important event associated with the development of NV. In addition, 50/10 rats that no longer demonstrated NV had a persistent subnormal retinal deltaPO(2), suggesting a continuing risk of development of additional retinal complications after resolution of the NV in ROP.
Wei Zhang; Yasuki Ito; Emily Berlin; Robin Roberts; Hongmei Luan; Bruce A Berkowitz
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Investigative ophthalmology & visual science     Volume:  44     ISSN:  0146-0404     ISO Abbreviation:  Invest. Ophthalmol. Vis. Sci.     Publication Date:  2003 Aug 
Date Detail:
Created Date:  2003-07-28     Completed Date:  2003-08-11     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  7703701     Medline TA:  Invest Ophthalmol Vis Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3551-5     Citation Subset:  IM    
Department of Anatomy and Cell Biology, Wayne State University, Detroit, Michigan, USA.
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MeSH Terms
Animals, Newborn
Carbon Dioxide
Disease Models, Animal
Hyperoxia / metabolism
Infant, Newborn
Magnetic Resonance Imaging
Oxygen / metabolism*
Oxygen Consumption*
Rats, Sprague-Dawley
Retina / metabolism*
Retinal Neovascularization / chemically induced,  diagnosis,  metabolism*
Retinopathy of Prematurity / chemically induced,  diagnosis,  metabolism*
Grant Support
Reg. No./Substance:
124-38-9/Carbon Dioxide; 7782-44-7/Oxygen; 8063-77-2/carbogen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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