| Specificity and mobility of biomacromolecular, multivalent constructs for cellular targeting. | |
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MedLine Citation:
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PMID: 18039007 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Effective targeting of drugs to cells requires that the drug reach the target cell and interact specifically with it. In this study, we synthesized a biomacromolecular, multivalent construct intended to target glioblastoma tumors. The construct was created by linking three dodecapeptides, reported to bind the alpha 6beta1 integrin, with poly(ethylene glycol) linkers. The construct is intended to be delivered locally, and it demonstrates a more homogeneous and more rapid perfusion profile in comparison with quantum dots. The binding specificity of the construct was investigated by using glioblastoma cells and normal human astrocyte cells. The results reveal qualitative differences in binding between glioma and normal human astrocyte cells, with a moderate increase in binding avidity due to multivalency (0.79 microM for the trivalent construct versus 4.28 microM for the dodecapeptide). Overall, biomacromolecular constructs appear to be a promising approach for targeting with high biocompatibility, good perfusion abilities, and specificity. |
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Authors:
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Elena V Rosca; Jill M Stukel; Robert J Gillies; Josef Vagner; Michael R Caplan |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2007-11-27 |
Journal Detail:
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Title: Biomacromolecules Volume: 8 ISSN: 1526-4602 ISO Abbreviation: Biomacromolecules Publication Date: 2007 Dec |
Date Detail:
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Created Date: 2007-12-11 Completed Date: 2008-02-28 Revised Date: 2013-06-06 |
Medline Journal Info:
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Nlm Unique ID: 100892849 Medline TA: Biomacromolecules Country: United States |
Other Details:
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Languages: eng Pagination: 3830-5 Citation Subset: IM |
Affiliation:
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Harrington Department of Bioengineering, Arizona State University, Tempe, Arizona 85287-9709, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Biocompatible Materials
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chemistry*,
metabolism* Cell Movement / physiology* Gene Targeting / methods Humans Macromolecular Substances / chemistry, metabolism Protein Binding / physiology Tumor Cells, Cultured |
| Grant Support | |
ID/Acronym/Agency:
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K22 DE014386/DE/NIDCR NIH HHS; K22 DE014846-01A1/DE/NIDCR NIH HHS; K22 DE014846-02/DE/NIDCR NIH HHS; K22 DE014846-03/DE/NIDCR NIH HHS; K22 DE014846-04/DE/NIDCR NIH HHS; R21 NS051310/NS/NINDS NIH HHS; R21 NS051310-01/NS/NINDS NIH HHS; R21 NS051310-02/NS/NINDS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Biocompatible Materials; 0/Macromolecular Substances |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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