Document Detail

Specificity and mobility of biomacromolecular, multivalent constructs for cellular targeting.
MedLine Citation:
PMID:  18039007     Owner:  NLM     Status:  MEDLINE    
Effective targeting of drugs to cells requires that the drug reach the target cell and interact specifically with it. In this study, we synthesized a biomacromolecular, multivalent construct intended to target glioblastoma tumors. The construct was created by linking three dodecapeptides, reported to bind the alpha 6beta1 integrin, with poly(ethylene glycol) linkers. The construct is intended to be delivered locally, and it demonstrates a more homogeneous and more rapid perfusion profile in comparison with quantum dots. The binding specificity of the construct was investigated by using glioblastoma cells and normal human astrocyte cells. The results reveal qualitative differences in binding between glioma and normal human astrocyte cells, with a moderate increase in binding avidity due to multivalency (0.79 microM for the trivalent construct versus 4.28 microM for the dodecapeptide). Overall, biomacromolecular constructs appear to be a promising approach for targeting with high biocompatibility, good perfusion abilities, and specificity.
Elena V Rosca; Jill M Stukel; Robert J Gillies; Josef Vagner; Michael R Caplan
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2007-11-27
Journal Detail:
Title:  Biomacromolecules     Volume:  8     ISSN:  1526-4602     ISO Abbreviation:  Biomacromolecules     Publication Date:  2007 Dec 
Date Detail:
Created Date:  2007-12-11     Completed Date:  2008-02-28     Revised Date:  2013-06-06    
Medline Journal Info:
Nlm Unique ID:  100892849     Medline TA:  Biomacromolecules     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3830-5     Citation Subset:  IM    
Harrington Department of Bioengineering, Arizona State University, Tempe, Arizona 85287-9709, USA.
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MeSH Terms
Biocompatible Materials / chemistry*,  metabolism*
Cell Movement / physiology*
Gene Targeting / methods
Macromolecular Substances / chemistry,  metabolism
Protein Binding / physiology
Tumor Cells, Cultured
Grant Support
K22 DE014386/DE/NIDCR NIH HHS; K22 DE014846-01A1/DE/NIDCR NIH HHS; K22 DE014846-02/DE/NIDCR NIH HHS; K22 DE014846-03/DE/NIDCR NIH HHS; K22 DE014846-04/DE/NIDCR NIH HHS; R21 NS051310/NS/NINDS NIH HHS; R21 NS051310-01/NS/NINDS NIH HHS; R21 NS051310-02/NS/NINDS NIH HHS
Reg. No./Substance:
0/Biocompatible Materials; 0/Macromolecular Substances

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