| SPECIFICITY OF IMPROVED METHODS FOR MYCOBACTIN BIOASSAY BY ARTHROBACTER TERREGENS. | |
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MedLine Citation:
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PMID: 14240956 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Antoine, Alan D. (Johns Hopkins University-Leonard Wood Memorial Leprosy Research Laboratory, Baltimore, Md.), Norman E. Morrison, and John H. Hanks. Specificity of improved methods for mycobactin bioassay by Arthrobacter terregens. J. Bacteriol. 88:1672-1677. 1964.-Arthrobacter terregens was used to assay mycobactin, a growth factor for Mycobacterium paratuberculosis. Improved techniques permit the assay of mycobactin within 3 to 4 days by agarplate or liquid-medium methods. For the agarplate method, Arthrobacter terregens gave linear increases in zonal growth at mycobactin concentrations of 0.07 to 0.30 mug per spot; for the liquid-medium method, linear increases in turbidimetric growth occurred at 0.05 to 0.27 mug/ml. Specificity studies show that the mycobactin hydrolytic products, cobactin and mycobactic acid, function as growth stimulators, but the high concentrations required would produce only minimal interference in mycobactin assays. Furthermore, the response to mycobactic acid is characterized by a delayed response of 3 days. Various synthetic hydroxylamine-containing compounds and metalchelating agents cannot replace the biological activity of mycobactin. Diacetylmycobactin is 7.4 times more effective than mycobactin as a growth stimulator. |
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Authors:
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A D ANTOINE; N E MORRISON; J H HANKS |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of bacteriology Volume: 88 ISSN: 0021-9193 ISO Abbreviation: J. Bacteriol. Publication Date: 1964 Dec |
Date Detail:
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Created Date: 1965-04-01 Completed Date: 1996-12-01 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 2985120R Medline TA: J Bacteriol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1672-7 Citation Subset: OM |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Arthrobacter* Biological Assay* Chelating Agents* Edetic Acid* Growth Substances* Hydroxylamines* Metabolism* Mycobacterium* Pharmacology* Research* |
| Chemical | |
Reg. No./Substance:
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0/Chelating Agents; 0/Growth Substances; 0/Hydroxylamines; 60-00-4/Edetic Acid |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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