Document Detail

Specificity of gossypol uncoupling: a comparative study of liver and spermatogenic cells.
MedLine Citation:
PMID:  3354655     Owner:  NLM     Status:  MEDLINE    
A comparative study of gossypol uncoupling of rat spermatogenic and liver cells shows that spermatogenic cells metabolizing pyruvate are two to three times more sensitive to gossypol uncoupling than either spermatogenic cells metabolizing glucose or liver cells metabolizing pyruvate or glucose. Direct measurements of in situ rat liver and spermatogenic cell mitochondrial respiration indicate that the pyruvate dependence of the gossypol uncoupling appears to be located in the spermatogenic cell mitochondria. A different type of mitochondrial uncoupler carbonyl cyanide m-chlorophenylhydrazone does not present the substrate-dependent uncoupling effect. This special interaction between spermatogenic cell pyruvate metabolism and gossypol uncoupling confers specificity to a bioenergetic model of gossypol action.
J Reyes; D J Benos
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Publication Detail:
Type:  Comparative Study; In Vitro; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The American journal of physiology     Volume:  254     ISSN:  0002-9513     ISO Abbreviation:  Am. J. Physiol.     Publication Date:  1988 Apr 
Date Detail:
Created Date:  1988-05-11     Completed Date:  1988-05-11     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0370511     Medline TA:  Am J Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  C571-6     Citation Subset:  IM    
Departamento de Fisiologia y Biofisica, Facultad de Medicina, Universidad de Chile, Santiago.
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MeSH Terms
Carbonyl Cyanide m-Chlorophenyl Hydrazone / pharmacology
Glucose / metabolism
Gossypol / pharmacology*
Liver / drug effects,  metabolism*
Mitochondria / drug effects,  metabolism*
Oxidative Phosphorylation / drug effects
Pyruvates / metabolism
Testis / drug effects,  metabolism*
Uncoupling Agents*
Grant Support
Reg. No./Substance:
0/Pyruvates; 0/Uncoupling Agents; 303-45-7/Gossypol; 50-99-7/Glucose; 555-60-2/Carbonyl Cyanide m-Chlorophenyl Hydrazone

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