| Specificity and functional characteristics of anti-HLA-A mAbs LGIII-147.4.1 and LGIII-220.6.2. | |
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MedLine Citation:
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PMID: 12889994 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Only three anti-HLA-A monoclonal antibodies (mAbs) have been described in the literature. Two of them recognize determinants shared by only a few HLA-A allospecificities. The third one, mAb 3G11, recognizes a determinant shared by most HLA-A allospecificities. Being an IgM, the latter mAb is not likely to be a useful probe in immunohistochemical reactions and in functional assays. Therefore, in the present study we have characterized the specificity of the mAbs LGIII-147.4.1 and LGIII-220.6.2. The two mAbs that do not share idiotypic determinants recognize distinct but spatially close antigenic determinants expressed on most of the gene products of the HLA-A locus. Specifically, the determinant recognized by mAb LGIII-220.6.2 is expressed on HLA-A1, -A2, -A3, -A26, -A28, -A29, -A30, -A33, -A36, -A74 and -A80 allospecificities. The determinant recognized by mAb LGIII-147.4.1, which appears to be located on the amino-acid residues 79-83 of the heavy chain, is expressed on all HLA-A allospecificities but HLA-A23, -A24, -A25 and -A32. Because of its broad reactivity, the mAb LGIII-147.4.1 was characterized in a number of assays. It was found to be a useful probe to measure the HLA-A antigen level in serum, to assess the HLA-A restriction of cytotoxic T lymphocytes (CTL) and to monitor HLA-A antigen expression in normal and malignant lesions. |
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Authors:
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X Wang; B Liang; V Rebmann; J Lu; E Celis; T Kageshita; H Grosse-Wilde; S Ferrone |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Tissue antigens Volume: 62 ISSN: 0001-2815 ISO Abbreviation: Tissue Antigens Publication Date: 2003 Aug |
Date Detail:
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Created Date: 2003-07-31 Completed Date: 2004-04-16 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0331072 Medline TA: Tissue Antigens Country: Denmark |
Other Details:
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Languages: eng Pagination: 139-48 Citation Subset: IM |
Affiliation:
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Department of Immunology, Roswell Park Cancer Institute, Buffalo, NY, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antibodies, Monoclonal / immunology*, physiology Enzyme-Linked Immunosorbent Assay HLA-A Antigens / immunology* Humans Hybridomas / immunology Melanoma / immunology, pathology Mice Skin / immunology, pathology Skin Neoplasms / immunology, pathology |
| Grant Support | |
ID/Acronym/Agency:
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P30 CA16056/CA/NCI NIH HHS; R01 CA67108/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Monoclonal; 0/HLA-A Antigens |
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