Document Detail

Specificity of the commonly used enzymatic assay for plasma cholesterol determination.
MedLine Citation:
PMID:  12401826     Owner:  NLM     Status:  MEDLINE    
AIM: To assess the specificity and sensitivity of the commonly used enzymatic colorimetric test for plasma cholesterol determination. METHODS: Interference with an enzymatic method for cholesterol measurement by several non-cholesterol sterols (beta sitosterol, campesterol, stigmasterol, stigmastanol, desmosterol, and lathosterol) was assessed. Some of these compounds are present in plasma at higher than normal concentrations either in rare genetic disorders, such as phytosterolaemia, or after the consumption of phytosterol enriched foods. RESULTS: The non-cholesterol sterols were detected by the assay in a linear manner. There was no competitive interference in the presence of cholesterol. CONCLUSIONS: This crossreactivity may affect the diagnosis and treatment of non-cholesterol dyslipidaemias, including phytosterolaemia and cerebrotendinous xanthomatosis. Similarly, changes in plasma lipid compositions after the consumption of phytosterol enriched foods cannot be specifically determined by this enzymatic assay. Until a more specific enzymatic assay is developed, alternative methods such as gas chromatography should be used to differentiate between cholesterol and non-cholesterol sterols.
M H Moghadasian; J J Frohlich; C H Scudamore
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of clinical pathology     Volume:  55     ISSN:  0021-9746     ISO Abbreviation:  J. Clin. Pathol.     Publication Date:  2002 Nov 
Date Detail:
Created Date:  2002-10-28     Completed Date:  2002-12-17     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0376601     Medline TA:  J Clin Pathol     Country:  England    
Other Details:
Languages:  eng     Pagination:  859-61     Citation Subset:  AIM; IM    
Department of Pathology and Laboratory Medicine, The University of British Columbia, Vancouver, Canada.
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MeSH Terms
Cholesterol / blood*
Cross Reactions
Hyperlipidemias / diagnosis*
Phytosterols / blood
Sensitivity and Specificity
Sterols / blood
Reg. No./Substance:
0/Phytosterols; 0/Sterols; 57-88-5/Cholesterol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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