| Specificity of the commonly used enzymatic assay for plasma cholesterol determination. | |
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MedLine Citation:
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PMID: 12401826 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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AIM: To assess the specificity and sensitivity of the commonly used enzymatic colorimetric test for plasma cholesterol determination. METHODS: Interference with an enzymatic method for cholesterol measurement by several non-cholesterol sterols (beta sitosterol, campesterol, stigmasterol, stigmastanol, desmosterol, and lathosterol) was assessed. Some of these compounds are present in plasma at higher than normal concentrations either in rare genetic disorders, such as phytosterolaemia, or after the consumption of phytosterol enriched foods. RESULTS: The non-cholesterol sterols were detected by the assay in a linear manner. There was no competitive interference in the presence of cholesterol. CONCLUSIONS: This crossreactivity may affect the diagnosis and treatment of non-cholesterol dyslipidaemias, including phytosterolaemia and cerebrotendinous xanthomatosis. Similarly, changes in plasma lipid compositions after the consumption of phytosterol enriched foods cannot be specifically determined by this enzymatic assay. Until a more specific enzymatic assay is developed, alternative methods such as gas chromatography should be used to differentiate between cholesterol and non-cholesterol sterols. |
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Authors:
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M H Moghadasian; J J Frohlich; C H Scudamore |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of clinical pathology Volume: 55 ISSN: 0021-9746 ISO Abbreviation: J. Clin. Pathol. Publication Date: 2002 Nov |
Date Detail:
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Created Date: 2002-10-28 Completed Date: 2002-12-17 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 0376601 Medline TA: J Clin Pathol Country: England |
Other Details:
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Languages: eng Pagination: 859-61 Citation Subset: AIM; IM |
Affiliation:
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Department of Pathology and Laboratory Medicine, The University of British Columbia, Vancouver, Canada. mhmoghad@interchange.ubc.ca |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Cholesterol
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blood* Colorimetry Cross Reactions Humans Hyperlipidemias / diagnosis* Phytosterols / blood Sensitivity and Specificity Sterols / blood |
| Chemical | |
Reg. No./Substance:
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0/Phytosterols; 0/Sterols; 57-88-5/Cholesterol |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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