Document Detail


Specificity of Ocimum basilicum geraniol synthase modified by its expression in different heterologous systems.
MedLine Citation:
PMID:  23108028     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Numerous aromatic plant species produce high levels of monoterpenols, using geranyl diphosphate (GPP) as a precursor. Sweet basil (Ocimum basilicum) geraniol synthase (GES) was used to evaluate the monoterpenol profiles arising from heterologous expressions in various plant models. Grapevine (Vitis vinifera) calli were transformed using Agrobacterium tumefasciens and the plants were regenerated. Thale cress (Arabidopsis thaliana) was transformed using the floral dip method. Tobacco (Nicotiana benthamiana) leaves were agro-infiltrated for transient expression. Although, as expected, geraniol was the main product detected in the leaves, different minor products were observed in these plants (V. vinifera: citronellol and nerol; N. benthamiana: linalool and nerol; A. thaliana: none). O. basilicum GES expression was also carried out with microbial system yeasts (Saccharomyces cerevisiae) and E. coli. These results suggest that the functional properties of a monoterpenol synthase depend not only on the enzyme's amino-acidic sequence, but also on the cellular background. They also suggest that some plant species or microbial expression systems could induce the simultaneous formation of several carbocations, and could thus have a natural tendency to produce a wider spectrum of monoterpenols.
Authors:
Marc J C Fischer; Sophie Meyer; Patricia Claudel; Mireille Perrin; Jean François Ginglinger; Claude Gertz; Jean E Masson; Danièle Werck-Reinhardt; Philippe Hugueney; Francis Karst
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-10-26
Journal Detail:
Title:  Journal of biotechnology     Volume:  -     ISSN:  1873-4863     ISO Abbreviation:  J. Biotechnol.     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-10-30     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8411927     Medline TA:  J Biotechnol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012. Published by Elsevier B.V.
Affiliation:
Univ Strasbourg, INRA, Inst Natl Recherche Agron, Métab Second Vigne, Unit Mixte Recherche Santé Vigne & Qual Vins, 28 rue de Herrlisheim, F-68021 Colmar, France. Electronic address: fischer@colmar.inra.fr.
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