| Specific translocation of tuftsin (Thr-Lys-Pro-Arg), a natural immunomodulating peptide, into the nuclei of human monocytes. | |
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MedLine Citation:
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PMID: 2494998 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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To delineate the mechanism of growth and differentiation activities of tuftsin (Thr-Lys-Pro-Arg), we examined the translocation of tuftsin after internalization by the target cells. We found using two independent techniques, fluorescence microscopy and autoradiography, that while in human polymorphonuclear leukocytes (terminally differentiated cells) the peptide remains in the cytoplasmic compartment, in monocytes it translocates to the nucleus. The ability of tuftsin to directly interact with DNA was documented by a large increase in the melting point of bovine DNA in the presence of tuftsin. It is suggested that the translocation, processing and action of tuftsin may depend on the differentiation state and/or on the type of effector cells. Also, tuftsin has the capacity to interact directly with DNA and, therefore, may have a potential for affecting gene activity. |
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Authors:
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J R Wagle; A T Ansevin; S E Dessens; K Nishioka |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Biochemical and biophysical research communications Volume: 159 ISSN: 0006-291X ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 1989 Mar |
Date Detail:
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Created Date: 1989-05-11 Completed Date: 1989-05-11 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1147-53 Citation Subset: IM |
Affiliation:
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Department of General Surgery, University of Texas M. D. Anderson Cancer Center, Houston 77030. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Autoradiography Cell Nucleus / metabolism* DNA / blood, drug effects Fluorescein-5-isothiocyanate Fluoresceins Fluorescent Dyes Humans Monocytes / metabolism* Neutrophils / metabolism* Nucleic Acid Denaturation Thiocyanates Tritium Tuftsin / blood*, pharmacology |
| Grant Support | |
ID/Acronym/Agency:
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CA45730/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Fluoresceins; 0/Fluorescent Dyes; 0/Thiocyanates; 10028-17-8/Tritium; 3326-32-7/Fluorescein-5-isothiocyanate; 9007-49-2/DNA; 9063-57-4/Tuftsin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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