Document Detail


Specific translocation of tuftsin (Thr-Lys-Pro-Arg), a natural immunomodulating peptide, into the nuclei of human monocytes.
MedLine Citation:
PMID:  2494998     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
To delineate the mechanism of growth and differentiation activities of tuftsin (Thr-Lys-Pro-Arg), we examined the translocation of tuftsin after internalization by the target cells. We found using two independent techniques, fluorescence microscopy and autoradiography, that while in human polymorphonuclear leukocytes (terminally differentiated cells) the peptide remains in the cytoplasmic compartment, in monocytes it translocates to the nucleus. The ability of tuftsin to directly interact with DNA was documented by a large increase in the melting point of bovine DNA in the presence of tuftsin. It is suggested that the translocation, processing and action of tuftsin may depend on the differentiation state and/or on the type of effector cells. Also, tuftsin has the capacity to interact directly with DNA and, therefore, may have a potential for affecting gene activity.
Authors:
J R Wagle; A T Ansevin; S E Dessens; K Nishioka
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  159     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  1989 Mar 
Date Detail:
Created Date:  1989-05-11     Completed Date:  1989-05-11     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1147-53     Citation Subset:  IM    
Affiliation:
Department of General Surgery, University of Texas M. D. Anderson Cancer Center, Houston 77030.
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MeSH Terms
Descriptor/Qualifier:
Autoradiography
Cell Nucleus / metabolism*
DNA / blood,  drug effects
Fluorescein-5-isothiocyanate
Fluoresceins
Fluorescent Dyes
Humans
Monocytes / metabolism*
Neutrophils / metabolism*
Nucleic Acid Denaturation
Thiocyanates
Tritium
Tuftsin / blood*,  pharmacology
Grant Support
ID/Acronym/Agency:
CA45730/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Fluoresceins; 0/Fluorescent Dyes; 0/Thiocyanates; 10028-17-8/Tritium; 3326-32-7/Fluorescein-5-isothiocyanate; 9007-49-2/DNA; 9063-57-4/Tuftsin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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