Document Detail

Specific thiol compounds complement deficiency in c-type cytochrome biogenesis in Escherichia coli carrying a mutation in a membrane-bound disulphide isomerase-like protein.
MedLine Citation:
PMID:  7957865     Owner:  NLM     Status:  MEDLINE    
Escherichia coli JCB606 carries a mutation in the dipZ gene, known to code for a disulphide isomerase-like protein, with the consequence that holo forms of neither exogenous nor endogenous c-type cytochromes are synthesised. This failure has been overcome by adding compounds containing thiol groups to the growth medium. Only L-cysteine and 2-mercaptoethane sulphonic acid were effective, suggesting a (stereo)specific binding site that could be occupied by these compounds in the absence of the catalytic domain of DipZ.
Y Sambongi; S J Ferguson
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  FEBS letters     Volume:  353     ISSN:  0014-5793     ISO Abbreviation:  FEBS Lett.     Publication Date:  1994 Oct 
Date Detail:
Created Date:  1994-11-29     Completed Date:  1994-11-29     Revised Date:  2009-09-29    
Medline Journal Info:
Nlm Unique ID:  0155157     Medline TA:  FEBS Lett     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  235-8     Citation Subset:  IM    
Department of Biochemistry, University of Oxford, UK.
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MeSH Terms
Alkaline Phosphatase / genetics,  metabolism
Base Sequence
Cell Fractionation
Culture Media
Cytochrome c Group / biosynthesis*,  genetics
Disulfides / metabolism
Escherichia coli / enzymology,  genetics*
Isomerases / genetics*,  metabolism
Molecular Sequence Data
Mutation / physiology*
Protein Disulfide-Isomerases
Recombinant Fusion Proteins / biosynthesis,  metabolism
Sulfhydryl Compounds / metabolism*
Grant Support
//Wellcome Trust
Reg. No./Substance:
0/Culture Media; 0/Cytochrome c Group; 0/Disulfides; 0/Recombinant Fusion Proteins; 0/Sulfhydryl Compounds; EC Phosphatase; EC 5.-/Isomerases; EC Disulfide-Isomerases

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