| Specific staining of glycine N-methyltransferase. | |
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MedLine Citation:
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PMID: 8586061 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Glycine N-methyltransferase from rabbit, human, rat and pig livers was separated by isoelectric focusing and a specific functional staining method was developed through the detection of sarcosine produced from the methylation of glycine. Isozyme patterns obtained in the various species tested differ both in the number of bands and apparent isoelectric points. These differences may explain the contradictory data on the subunit structure and glycosylation status of the enzyme reported so far. |
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Authors:
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F Santos; A Amorim; J Kömpf |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Electrophoresis Volume: 16 ISSN: 0173-0835 ISO Abbreviation: Electrophoresis Publication Date: 1995 Oct |
Date Detail:
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Created Date: 1996-03-22 Completed Date: 1996-03-22 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8204476 Medline TA: Electrophoresis Country: GERMANY |
Other Details:
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Languages: eng Pagination: 1898-9 Citation Subset: IM |
Affiliation:
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Instituto de Antropologia, Faculdade de Ciências, Universidade do Porto, Portugal. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Glycine / metabolism Glycine N-Methyltransferase Humans Isoelectric Focusing / methods* Isoenzymes / isolation & purification*, metabolism Liver / enzymology* Methylation Methyltransferases / isolation & purification*, metabolism Rabbits Rats Sarcosine / analysis, metabolism Staining and Labeling* Swine |
| Chemical | |
Reg. No./Substance:
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0/Isoenzymes; 107-97-1/Sarcosine; 56-40-6/Glycine; EC 2.1.1.-/Methyltransferases; EC 2.1.1.20/Glycine N-Methyltransferase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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