Document Detail


Specific and sensitive enzyme-linked immunosorbent assays for analysis of residual allergenic food proteins in commercial bottled wine fined with egg white, milk, and nongrape-derived tannins.
MedLine Citation:
PMID:  18163561     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Regulations introduced by the Food Standards Australia New Zealand in December 2002 require all wine and wine product labels in Australia to identify the presence of a processing aid, additive or other ingredient, which is known to be a potential allergen. The objective of this study was to establish sensitive assays to detect and measure allergenic proteins from commonly used processing aids in final bottled wine. Sensitive and specific enzyme-linked immunosorbent assays (ELISA) were developed and established for the proteins casein, ovalbumin, and peanut. Lower limits of detection of these proteins were 8, 1, and 8 ng/mL, respectively. A panel of 153 commercially available bottled Australian wines were tested by these ELISA, and except for two red wines known to contain added whole eggs, residuals of these food allergens were not detected in any wine. These findings are consistent with a lack of residual potentially allergenic egg-, milk-, or nut-derived processing aids in final bottled wine produced in Australia according to good manufacturing practice at a concentration that could cause an adverse reaction in egg, milk, or peanut/tree-nut allergic adult consumers.
Authors:
Jennifer M Rolland; Effie Apostolou; Maria P de Leon; Creina S Stockley; Robyn E O'Hehir
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-12-29
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  56     ISSN:  0021-8561     ISO Abbreviation:  J. Agric. Food Chem.     Publication Date:  2008 Jan 
Date Detail:
Created Date:  2008-01-17     Completed Date:  2008-04-08     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  349-54     Citation Subset:  IM    
Affiliation:
Department of Immunology, Monash University, The Alfred, Commercial Road, Melbourne, Victoria, Australia 3004. jennifer.rolland@med.monash.edu.au
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MeSH Terms
Descriptor/Qualifier:
Allergens / analysis*
Antibodies, Monoclonal
Arachis hypogaea / chemistry,  immunology
Caseins / analysis*
Drug Residues / analysis
Enzyme-Linked Immunosorbent Assay / methods*
Food Handling / methods
Ovalbumin / analysis*
Sensitivity and Specificity
Tannins / analysis*
Wine / analysis*
Chemical
Reg. No./Substance:
0/Allergens; 0/Antibodies, Monoclonal; 0/Caseins; 0/Tannins; 9006-59-1/Ovalbumin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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