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Specific and sensitive detection of Alcaligenes species from an agricultural environment.
MedLine Citation:
PMID:  23323750     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
We developed a quantitative real-time PCR assay to specificity detect and quantify the genus Alcaligenes in samples from the agricultural environment, such as vegetables and farming soils. The minimum detection sensitivity was 106 fg of pure culture DNA, corresponding to DNA extracted from two cells of A. faecalis. To evaluate the detection limit of A. faecalis, serially diluted genomic DNA from A. faecalis was mixed with DNA extracted from soil and vegetables, and then a standard curve was constructed. These results indicated that Alcaligenes species are present in the plant phytosphere at levels 10(2) -10(4) times lower than those in soil. Our approach will be useful for tracking or quantifying species of the genus Alcaligenes in the agricultural environment.
Authors:
Miyo Nakano; Masumi Niwa; Norihiro Nishimura
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-17
Journal Detail:
Title:  Microbiology and immunology     Volume:  -     ISSN:  1348-0421     ISO Abbreviation:  Microbiol. Immunol.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-17     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7703966     Medline TA:  Microbiol Immunol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2013 The Societies and Blackwell Publishing Asia Pty Ltd.
Affiliation:
Department of Translational Medical Science and Molecular and Cellular Pharmacology, Pharmacogenomics, and Pharmacoinformatics, Mie University Graduate School of Medicine, Mie University, 2-174 Edobashi, Tsu, Mie 514-8507, Japan.
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