| Specific, saturable binding and uptake of rat chylomicron remnants by rat skin fibroblasts. | |
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MedLine Citation:
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PMID: 7097128 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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To investigate the possible contribution of chylomicron remnants to the accumulation of cholesterol in non-hepatic tissues, rat chylomicron remnants were incubated with rat skin fibroblasts. The binding of remnants was saturable and specific. Native, undegraded chylomicrons were almost as effective as unlabeled remnants in displacing the uptake of labeled remnants. Rat low density and high density lipoproteins were relatively ineffective in displacing the uptake of labeled remnants. Accumulation of radioactive unesterified fatty acids occurred in proportion to the uptake of labeled remnants, indicating probable internalization and degradation of the particles after binding. The incorporation of added [14C]acetate into cell non-saponifiable lipids was not significantly suppressed by added remnants, indicating an apparent lack of feedback regulation of cholesterol biosynthesis after remnant uptake. Our results show that the physiological mechanism underlying uptake of remnants by hepatic parenchymal cells might not be accounted for by tissue or cellular specificity, but may perhaps arise because of the lower capillary permeability of extra-hepatic sites compared with the hepatic sinusoid. |
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Authors:
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T G Redgrave; N H Fidge; J Yin |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of lipid research Volume: 23 ISSN: 0022-2275 ISO Abbreviation: J. Lipid Res. Publication Date: 1982 May |
Date Detail:
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Created Date: 1982-09-10 Completed Date: 1982-09-10 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0376606 Medline TA: J Lipid Res Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 638-44 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Apolipoproteins / metabolism Binding, Competitive Chylomicrons / metabolism* Fibroblasts / metabolism Humans Kinetics Lipoproteins, HDL / metabolism Lipoproteins, LDL / metabolism Male Rats Rats, Inbred Strains Skin / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Apolipoproteins; 0/Chylomicrons; 0/Lipoproteins, HDL; 0/Lipoproteins, LDL |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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