Document Detail


Specific rosette formation between fibroblasts and erythrocytes.
MedLine Citation:
PMID:  7033257     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Fibroblast cells (BHK-21, Swiss 3T3, SV403T3, L929) will form rosettes by binding to erythrocytes of specific types. The specificity of binding varies with the cell line. Monolayer cultures of spread baby hamster kidney (BHK) cells also bind erythrocytes with the same specificity exhibited by suspended cells. Our studies have concentrated on rosette formation between trypsinzed-ox (TOx) erythrocytes and BHK cells. Extensive protease treatment of the BHK cells reduces their ability to bind TOx erythrocytes in a dose- and time-dependent manner, suggesting the involvement of protein in the binding site. Simple saccharides, and glycopeptide isolated from ox erythrocyte ghosts, do not inhibit rosetting. However, neutral glycosphinogolipids from ox erythrocyte ghosts inhibit BHK-TOx rosetting, whereas neutral glycosphingolipids from non-rosetting rabbit erythrocytes have no effect on rosetting. Furthermore, incorporation of ox glycolipids into guinea-pig erythrocytes causes these non-rosetting erythrocytes to adhere to BHK cells. These experiments suggest that specific glycolipids of the ox erythrocyte act as receptors for binding sites on the cell surface of BHK cells. These cell-specific binding sites may play a role in cell-surface events involving carbohydrate recognition.
Authors:
S D Rosen; M S Singer; C G Glabe; L B Grabel
Related Documents :
7758467 - Introduction of specific carbohydrates into eucalyptus gunnii cells increases their fre...
7709027 - Viral haemagglutination of glutaraldehyde-fixed sheep erythrocytes.
21173377 - Metastatic potential of cancer stem cells in head and neck squamous cell carcinoma.
10924267 - Green fluorescent protein: a novel viability assay for cryobiological applications.
3075607 - Intrinsic factor in the human fetal stomach. an immunocytochemical study.
20811567 - Inherited adaptation of genome-rewired cells in response to a challenging environment.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of cell science     Volume:  51     ISSN:  0021-9533     ISO Abbreviation:  J. Cell. Sci.     Publication Date:  1981 Oct 
Date Detail:
Created Date:  1982-03-13     Completed Date:  1982-03-13     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0052457     Medline TA:  J Cell Sci     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  279-94     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Binding Sites
Cattle
Cell Line
Cricetinae
Erythrocytes / physiology*
Fibroblasts / physiology*
Guinea Pigs
Kidney / cytology
Peptide Hydrolases / pharmacology
Rabbits
Rats
Rosette Formation
Grant Support
ID/Acronym/Agency:
CA06668/CA/NCI NIH HHS; GM00322/GM/NIGMS NIH HHS; GM23547/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
EC 3.4.-/Peptide Hydrolases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  BS I-B4 isolectin as a probe for an investigation of membrane alterations and transformation phenoty...
Next Document:  High-resolution electron microscopy of glycoproteins: the crystalline cell wall of Lobomonas.