Document Detail

Specific rosette formation between fibroblasts and erythrocytes.
MedLine Citation:
PMID:  7033257     Owner:  NLM     Status:  MEDLINE    
Fibroblast cells (BHK-21, Swiss 3T3, SV403T3, L929) will form rosettes by binding to erythrocytes of specific types. The specificity of binding varies with the cell line. Monolayer cultures of spread baby hamster kidney (BHK) cells also bind erythrocytes with the same specificity exhibited by suspended cells. Our studies have concentrated on rosette formation between trypsinzed-ox (TOx) erythrocytes and BHK cells. Extensive protease treatment of the BHK cells reduces their ability to bind TOx erythrocytes in a dose- and time-dependent manner, suggesting the involvement of protein in the binding site. Simple saccharides, and glycopeptide isolated from ox erythrocyte ghosts, do not inhibit rosetting. However, neutral glycosphinogolipids from ox erythrocyte ghosts inhibit BHK-TOx rosetting, whereas neutral glycosphingolipids from non-rosetting rabbit erythrocytes have no effect on rosetting. Furthermore, incorporation of ox glycolipids into guinea-pig erythrocytes causes these non-rosetting erythrocytes to adhere to BHK cells. These experiments suggest that specific glycolipids of the ox erythrocyte act as receptors for binding sites on the cell surface of BHK cells. These cell-specific binding sites may play a role in cell-surface events involving carbohydrate recognition.
S D Rosen; M S Singer; C G Glabe; L B Grabel
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of cell science     Volume:  51     ISSN:  0021-9533     ISO Abbreviation:  J. Cell. Sci.     Publication Date:  1981 Oct 
Date Detail:
Created Date:  1982-03-13     Completed Date:  1982-03-13     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0052457     Medline TA:  J Cell Sci     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  279-94     Citation Subset:  IM    
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MeSH Terms
Binding Sites
Cell Line
Erythrocytes / physiology*
Fibroblasts / physiology*
Guinea Pigs
Kidney / cytology
Peptide Hydrolases / pharmacology
Rosette Formation
Grant Support
Reg. No./Substance:
EC 3.4.-/Peptide Hydrolases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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