| Specific requirements for elements of the 5' and 3' terminal regions in flavivirus RNA synthesis and viral replication. | |
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MedLine Citation:
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PMID: 18234265 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We initially studied requirements for 5' and 3' terminal regions (TRs) in flavivirus negative strand synthesis in vitro. Purified West Nile (WNV) and dengue-2 (DV2) RNA polymerases were both active with all-WNV or all-DV2 subgenomic RNAs containing the 5'- and 3'TRs of the respective genomes. However, subgenomic RNAs in which the 5'-noncoding region (5'NCR) or the 5'ORF (nts 100-230) in the 5'TR were substituted by analogous sequences derived from the heterologous genome were modestly to severely defective as templates for either polymerase. We also evaluated the infectivity of substitution mutant WNV genome-length RNAs. All WNV RNAs containing the DV2 3'SL were unable to replicate. However, WNV RNAs containing substitutions of the 5'NCR, the capsid gene, and/or 3'NCR nt sequences upstream from the WNV 3'SL, by the analogous DV2 nt sequences, were infectious. Combined results suggested that replication was not dependent upon species homology between the 3'SL and NS5. |
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Authors:
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Li Yu; Masako Nomaguchi; R Padmanabhan; Lewis Markoff |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2008-01-29 |
Journal Detail:
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Title: Virology Volume: 374 ISSN: 0042-6822 ISO Abbreviation: Virology Publication Date: 2008 Apr |
Date Detail:
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Created Date: 2008-04-14 Completed Date: 2008-05-22 Revised Date: 2013-03-27 |
Medline Journal Info:
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Nlm Unique ID: 0110674 Medline TA: Virology Country: United States |
Other Details:
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Languages: eng Pagination: 170-85 Citation Subset: IM |
Affiliation:
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Laboratory of Vector-Borne Virus Diseases, Division of Viral Products, Office of Vaccines Research and Review, CBER, FDA, Bethesda, Maryland, USA. yuli@cber.fda.gov |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Base Sequence Cell Line Cercopithecus aethiops Culicidae Dengue Virus / genetics*, physiology Models, Molecular Molecular Sequence Data Nucleic Acid Conformation RNA Replicase / metabolism RNA, Untranslated / genetics* RNA, Viral / biosynthesis, genetics* Regulatory Sequences, Nucleic Acid* Viral Plaque Assay Viral Proteins / metabolism Virus Replication / physiology* West Nile virus / genetics*, physiology |
| Grant Support | |
ID/Acronym/Agency:
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AI32078/AI/NIAID NIH HHS; R01 AI032078-08/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/RNA, Untranslated; 0/RNA, Viral; 0/Viral Proteins; EC 2.7.7.48/RNA Replicase |
| Comments/Corrections | |
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