| Specific repression of rat prolactin gene expression in transplanted tumor cells. | |
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MedLine Citation:
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PMID: 8152427 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Transplantation of GH3 rat pituitary tumor cells that express both PRL and GH to female Wistar-Furth rats results in tumors that secrete only GH. We have used in vivo passage of GH3 cells as a model system to study specific repression of PRL. RNA blot hybridization revealed that PRL message was repressed 95% in cells transplanted to host animals compared to that in GH3 cells in culture. In contrast, there was little change in GH message in the transplanted cells, and there was a 4-fold increase in insulin-like growth factor-I transcript levels. When the transplanted cells were returned to cell culture, PRL mRNA levels increased rapidly, reaching levels similar to those in GH3 cells within 72 h. Gene transfer studies demonstrated a low level PRL promoter utilization in GH3 cells after in vivo passage, when endogenous PRL was repressed. Transfection of the transplanted cells maintained in culture for 96 h, when endogenous PRL was expressed, demonstrated increased PRL promoter activity. Messenger RNA levels for the transcription factor Pit-1 were equivalent in GH3 cells and cells after in vivo passage, and the presence of Pit-1 protein in extracts from transplanted cells was demonstrated by Western blot analysis. Electrophoretic gel mobility shift assays indicated that protein interactions with the PRL promoter were very different for extracts prepared from cells in which PRL was repressed compared to those from cells maintained in culture until PRL expression had recovered.(ABSTRACT TRUNCATED AT 250 WORDS) |
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Authors:
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R N Day; K H Day |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Molecular endocrinology (Baltimore, Md.) Volume: 8 ISSN: 0888-8809 ISO Abbreviation: Mol. Endocrinol. Publication Date: 1994 Jan |
Date Detail:
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Created Date: 1994-05-09 Completed Date: 1994-05-09 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8801431 Medline TA: Mol Endocrinol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 12-20 Citation Subset: IM |
Affiliation:
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Department of Medicine, University of Virginia, Charlottesville 22908. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Base Sequence Binding, Competitive Blotting, Western DNA / metabolism DNA-Binding Proteins / metabolism Female Gene Expression Regulation, Neoplastic* Insulin-Like Growth Factor I / genetics Molecular Sequence Data Neoplasm Transplantation Nucleic Acid Hybridization Pituitary Neoplasms / genetics* Prolactin / genetics* RNA, Messenger / analysis, metabolism Rats Rats, Inbred WF Transcription Factor Pit-1 Transcription Factors / metabolism Transfection Tumor Cells, Cultured |
| Chemical | |
Reg. No./Substance:
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0/DNA-Binding Proteins; 0/Pou1f1 protein, rat; 0/RNA, Messenger; 0/Transcription Factor Pit-1; 0/Transcription Factors; 67763-96-6/Insulin-Like Growth Factor I; 9002-62-4/Prolactin; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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