| Specific receptors for platelet-derived growth factor on cells derived from connective tissue and glia. | |
| | |
MedLine Citation:
|
PMID: 6943569 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
A cellular receptor for platelet-derived growth factor (PDGF) was demonstrated by incubation of 125I-labeled PDGF with human foreskin fibroblast cultures followed by liberation of cell-bound radioactivity with Triton X-100. The cellular binding of labeled PDGF in the presence of increasing amounts of unlabeled PDGF showed saturation; Scatchard analysis of binding data indicated a single class of receptors having kd = 1 X 10(-9) M. The number of PDGF binding sites was approximately 3 X 10(5)/cell. Labeled PDGF binding reached an apparent equilibrium after 3 hr at 4 degrees C. At 37 degrees C, it passed a maximum after 30 min and then decreased with time due to degradation of the tracer. A large excess of unlabeled PDGF reduced labeled PDGF binding by more than 90% whereas similar doses of epidermal growth factor, fibroblast growth factor, or insulin had no effect. It was concluded that PDGF did not share receptors with these factors. PDGF receptors were found on skin fibroblasts, normal and malignant glial cells, smooth muscle cells, and 3T3 cells but not on epithelial-derived cells, neuroblastoma cells, endothelial cells, or peripheral lymphocytes.l As only the receptor-positive cells--i.e., the connective tissue- and glia-derived cells--are responsive to stimulation with PDGF, these findings imply a functional significance of the PDGF receptor. |
| | |
Authors:
|
C H Heldin; B Westermark; A Wasteson |
Related Documents
:
|
14981939 - Growth factor-enhanced expression and activity of matrix metalloproteases in human non-... 11144969 - Inducing proliferation of human amniotic epithelial (hae) cells for cell therapy. 12060069 - Planarian fibroblast growth factor receptor homologs expressed in stem cells and cephal... 7780129 - Interleukin-10 is an autocrine growth factor for acquired immunodeficiency syndrome-rel... 7626139 - Androgen induction of follicular epithelial cell growth is mediated via insulin-like gr... 2992919 - Insulin-like growth factors as intraovarian regulators of granulosa cell growth and fun... 6230459 - Non-targeted mutagenesis of unirradiated lambda phage in escherichia coli host cells ir... 6605739 - The binding of human salivary alpha-amylase by oral strains of streptococcal bacteria. 23316279 - Csn5 specifically interacts with cdk2 and controls senescence in a cytoplasmic cyclin e... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 78 ISSN: 0027-8424 ISO Abbreviation: Proc. Natl. Acad. Sci. U.S.A. Publication Date: 1981 Jun |
Date Detail:
|
Created Date: 1981-10-29 Completed Date: 1981-10-29 Revised Date: 2009-11-18 |
Medline Journal Info:
|
Nlm Unique ID: 7505876 Medline TA: Proc Natl Acad Sci U S A Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 3664-8 Citation Subset: IM |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Blood Platelets Cells, Cultured Connective Tissue / metabolism* Fibroblasts / metabolism Growth Substances / metabolism* Humans Kinetics Mice Mitogens Neuroglia / metabolism* Peptides / metabolism* Platelet-Derived Growth Factor Receptors, Mitogen / metabolism* Temperature Tissue Distribution |
| Chemical | |
Reg. No./Substance:
|
0/Growth Substances; 0/Mitogens; 0/Peptides; 0/Platelet-Derived Growth Factor; 0/Receptors, Mitogen |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Correlation of double-minute chromosomes with unstable multidrug cross-resistance in uptake mutants ...
Next Document: Attachment of smooth muscle cells to collagen and their migration toward platelet-derived growth fac...