Document Detail


Specific receptors for epidermal growth factor in rat intestinal microvillus membranes.
MedLine Citation:
PMID:  3348408     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Epidermal growth factor (EGF) is present in high concentrations in milk, salivary, and pancreaticobiliary secretions. EGF, delivered to the intestinal lumen by these fluids, appears to influence intestinal proliferation. Because EGF exerts its mitogenic effect through binding to specific membrane-bound receptors, binding studies of 125I-labeled EGF to purified microvillus membrane (MVM) preparations from fetal, newborn, and adult rat small intestine were performed. Using the membrane filter technique, binding of 125I-EGF to adult MVM was specific, saturable, and reversible. Adult and fetal MVM binding was rapid and reached a plateau after 30 min at both 20 and 37 degrees C. No binding was detected at 4 degrees C. Specific binding increased linearly from 0 to 75 micrograms MVM protein. Scatchard analysis revealed a single class of receptors in fetal and adult MVM with an association constant of 1.0 +/- 0.35 X 10(9) and 2.3 +/- 1.6 X 10(9) M-1, respectively. Binding capacity was 435.0 +/- 89 and 97.7 +/- 41.3 fmol 125I-EGF bound/mg MVM protein for fetal and adult MVM, respectively. Newborn MVM binding was negligible. After binding, cross-linking utilizing disuccinimidyl suberate, and sodium dodecyl sulfate-polyacrylamide gel electrophoresis, autoradiography revealed a 170-kDa receptor. These data demonstrate specific receptors for EGF on MVM of rat small intestine and, thus, suggest a mechanism for the intraluminal regulation of enterocyte proliferation by EGF.
Authors:
J F Thompson
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The American journal of physiology     Volume:  254     ISSN:  0002-9513     ISO Abbreviation:  Am. J. Physiol.     Publication Date:  1988 Mar 
Date Detail:
Created Date:  1988-04-21     Completed Date:  1988-04-21     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0370511     Medline TA:  Am J Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  G429-35     Citation Subset:  IM    
Affiliation:
Department of Pediatrics, New England Medical Center, Tufts University School of Medicine, Boston, Massachusetts 02111.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cross-Linking Reagents / diagnostic use
Intestines / metabolism*,  ultrastructure
Membranes / metabolism
Microvilli / metabolism
Rats
Rats, Inbred Strains
Receptor, Epidermal Growth Factor / metabolism*
Grant Support
ID/Acronym/Agency:
1 P30 AM-34928-02/AM/NIADDK NIH HHS
Chemical
Reg. No./Substance:
0/Cross-Linking Reagents; EC 2.7.10.1/Receptor, Epidermal Growth Factor

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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