| Specific parasitism of purified vaginal epithelial cells by Trichomonas vaginalis. | |
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MedLine Citation:
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PMID: 3262088 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Human vaginal epithelial cells (VECs) from vaginal swabs obtained from normal women or from patients with trichomoniasis were purified, and VEC parasitism by Trichomonas vaginalis was examined. Trichomonads bound equally well to live or dead VECs, and up to 20% of VECs were parasitized. Trichomonal cytadherence of human VECs was time, temperature, and pH dependent. Saturation binding levels of live trichomonads to VECs gave approximately 2 organisms adherent to parasitized VEC. No differences in cytadherence levels were detected by different isolates to VECs from the same patient compared with adherence to VECs from normal individuals. Trypsinized, live T. vaginalis organisms failed to recognize VECs. A ligand assay identified four adhesin candidates, and only organisms without a prominent immunogen on the surface (negative phenotype) cytadhered to VECs and synthesized the adhesins, confirming the results of a recently published report by us on adherence to HeLa cell monolayers (J. F. Alderete and G. E. Garza, Infect. Immun. 56:28-33, 1988). These data show the ability of T. vaginalis to parasitize human vaginal epithelial cells in a specific receptor-ligand manner. |
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Authors:
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J F Alderete; P Demeś; A Gombosova; M Valent; M Fabusová; A Jánoska; J Stefanovic; R Arroyo |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Infection and immunity Volume: 56 ISSN: 0019-9567 ISO Abbreviation: Infect. Immun. Publication Date: 1988 Oct |
Date Detail:
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Created Date: 1988-10-26 Completed Date: 1988-10-26 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 0246127 Medline TA: Infect Immun Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 2558-62 Citation Subset: IM |
Affiliation:
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Department of Microbiology, University of Texas Health Science Center, San Antonio 78284. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antigens, Protozoan / immunology Antigens, Surface / metabolism Cell Adhesion Cell Membrane / metabolism Epithelium / parasitology Female Humans Time Factors Trichomonas Vaginitis / parasitology* Trichomonas vaginalis Vagina / cytology, parasitology* |
| Grant Support | |
ID/Acronym/Agency:
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AI-18768/AI/NIAID NIH HHS; K04-A1-00584//PHS HHS |
| Chemical | |
Reg. No./Substance:
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0/Antigens, Protozoan; 0/Antigens, Surface |
| Comments/Corrections | |
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