Document Detail

Specific and nonspecific interactions of integration host factor with oligo DNAs as revealed by circular dichroism spectroscopy and filter binding assay.
MedLine Citation:
PMID:  1586158     Owner:  NLM     Status:  MEDLINE    
Binding specificity of integration host factor (IHF) to oligo DNAs has been studied by circular dichroism (CD) spectroscopy and filter binding experiment. CD difference spectra of IHF-DNA complexes demonstrated that a conformational change in DNA was induced by binding of IHF when DNA had a consensus sequence for the binding sites of IHF, but that such conformational change was not observed for consensus DNA 20 mer as well as nonconsensus DNA 45 mer. Dissociation constants for IHF-DNA complexes determined by filter binding assay showed that IHF has indeed stronger affinity to DNA with the consensus binding site than to nonconsensus DNA, but the difference in its affinity between consensus and nonconsensus DNAs was rather small, 3.4-fold. It was, therefore, concluded that the flanking regions of the consensus sequence are important for the specific binding of IHF and that its binding specificity is well characterized by the induced conformational change in DNA rather than by dissociation constants for IHF-DNA complexes.
H Kurumizaka; F Kanke; U Matsumoto; H Shindo
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Archives of biochemistry and biophysics     Volume:  295     ISSN:  0003-9861     ISO Abbreviation:  Arch. Biochem. Biophys.     Publication Date:  1992 Jun 
Date Detail:
Created Date:  1992-06-16     Completed Date:  1992-06-16     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372430     Medline TA:  Arch Biochem Biophys     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  297-301     Citation Subset:  IM    
Tokyo College of Pharmacy, Japan.
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MeSH Terms
Bacterial Proteins / metabolism*
Base Sequence
Circular Dichroism
DNA / chemistry,  metabolism*
DNA-Binding Proteins / metabolism*
Integration Host Factors
Molecular Sequence Data
Nucleic Acid Conformation
Substrate Specificity
Reg. No./Substance:
0/Bacterial Proteins; 0/DNA-Binding Proteins; 0/Integration Host Factors; 9007-49-2/DNA

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