Document Detail

Specific movement of cell membranes fused with HVJ (Sendai virus).
MedLine Citation:
PMID:  4365583     Owner:  NLM     Status:  MEDLINE    
When Ehrlich ascites tumor cells fused with UV-inactivated HVJ (Sendai virus) were cultured, the viral envelope antigens, which had been integrated at random into the cell membranes, accumulated at one site on the fused cell. The site showed a specific structure, namely, a projection of the cytoplasm from the spherical fused cell surface with clusters of many long microvillilike protrusions from it. When the fused cells were conjugated with antibody against Ehrlich ascites tumor cells and cultured, the antigen-antibody complex also accumulated on the projection.Associated with the development of the projection and accumulation of viral antigens, vacuoles developed at the base of the projection in the cytoplasm of the fused cells in culture. The development of the projection and vacuoles and the accumulation of viral antigens were all inhibited by glucose, mannose, galactose, sodium azide, and cycloheximide, and these inhibitions were reversible. All these structures were observed in multinucleated cells, but few in mononucleated cells after reaction with HVJ (Sendai virus). We consider that these three phenomena correlate with each other and may result from changes in cell-membrane systems caused by fusion of cells.
Y Okada; J Kim; Y Maeda; I Koseki
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  71     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  1974 May 
Date Detail:
Created Date:  1974-08-23     Completed Date:  1974-08-23     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2043-7     Citation Subset:  IM    
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MeSH Terms
Antigens, Viral / analysis
Carcinoma, Ehrlich Tumor
Cell Fusion*
Cell Membrane / immunology,  microbiology*
Cells, Cultured
Fluorescent Antibody Technique
Parainfluenza Virus 1, Human* / immunology
Reg. No./Substance:
0/Antigens, Viral

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