Document Detail


Specific members of the Jun protein family regulate collagenase expression in response to various extracellular stimuli.
MedLine Citation:
PMID:  1336108     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The transcription factor AP1 which regulates expression of collagenase in response to various extracellular signals is a multimeric complex composed of members of the Jun- and Fos families. To examine the biological role of the various components in signal transduction we analyzed the expression of two of them (cJun, JunB) and collagenase in response to phorbol esters, cAMP and TGF-beta. While all three genes are induced by phorbol ester and TGF-beta only JunB is induced by cAMP. In contrast expression of cJun and collagenase is reduced by cAMP indicating that cJun and JunB are not coordinately regulated. In addition JunB is not an efficient activator of the cJun and collagenase promoters although both cJun and JunB exhibit similar DNA binding properties, indicating that the differences in biological activity is due to differences in their activation domains. Our results imply that enhanced expression of collagenase (and cJun) depends on the activation of cJun. Expression of cJun and collagenase is inhibited under certain conditions of high levels of JunB. This suggests a negative regulatory function of JunB which greatly expands the potential of the Jun protein family in changing the transcription of specific genes involved in triggering complex biological processes.
Authors:
P Angel; M Karin
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Matrix (Stuttgart, Germany). Supplement     Volume:  1     ISSN:  0940-1199     ISO Abbreviation:  Matrix Suppl     Publication Date:  1992  
Date Detail:
Created Date:  1993-02-05     Completed Date:  1993-02-05     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  9312140     Medline TA:  Matrix Suppl     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  156-64     Citation Subset:  IM    
Affiliation:
Kernforschungszentrum Karlsruhe, Institut für Genetik, FRG.
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MeSH Terms
Descriptor/Qualifier:
Collagenases / biosynthesis*
Cyclic AMP / physiology
DNA, Neoplasm / metabolism
Enzyme Induction / drug effects
Forskolin / pharmacology
Gene Expression Regulation / drug effects
Genes, fos
Genes, jun
Humans
Neoplasm Proteins / biosynthesis
Promoter Regions, Genetic
Protein Multimerization
Proto-Oncogene Proteins c-fos / biosynthesis,  physiology
Proto-Oncogene Proteins c-jun / biosynthesis,  physiology*
Signal Transduction
Tetradecanoylphorbol Acetate / pharmacology
Transforming Growth Factor beta / pharmacology
Tumor Cells, Cultured
Chemical
Reg. No./Substance:
0/DNA, Neoplasm; 0/Neoplasm Proteins; 0/Proto-Oncogene Proteins c-fos; 0/Proto-Oncogene Proteins c-jun; 0/Transforming Growth Factor beta; 16561-29-8/Tetradecanoylphorbol Acetate; 60-92-4/Cyclic AMP; 66428-89-5/Forskolin; EC 3.4.24.-/Collagenases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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