| Specific lysis of noradrenergic synaptosomes by an antiserum to dopamine-beta-hydroxylase. | |
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MedLine Citation:
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PMID: 2873056 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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An antiserum to dopamine-beta-hydroxylase purified from bovine adrenal medulla, acting in the presence of complement, caused the release of 12% of lactate dehydrogenase, 20% of tyrosine hydroxylase activity, and 40% of noradrenaline (NA) content from synaptosomes prepared from rat brain cerebral cortex. Uptake of [3H]NA was reduced by 54%. Anti-serum alone or complement alone were without action. The antiserum plus complement had no effect on choline uptake and did not release choline acetyltransferase, glutamate decarboxylase, dopamine or 5-hydroxytryptamine. These results suggest selective lysis of noradrenergic terminals had occurred. An enhancement of lysis was not observed when synaptosomes were stimulated with 75 mequiv./lK+ and exposed to a sub-maximal dose of antiserum, plus complement. |
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Authors:
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M Docherty; H F Bradford; T H Joh |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: FEBS letters Volume: 202 ISSN: 0014-5793 ISO Abbreviation: FEBS Lett. Publication Date: 1986 Jun |
Date Detail:
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Created Date: 1986-07-30 Completed Date: 1986-07-30 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0155157 Medline TA: FEBS Lett Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 37-40 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cerebral Cortex / enzymology Complement System Proteins / pharmacology Dopamine / metabolism Dopamine beta-Hydroxylase / immunology* Immune Sera / pharmacology* L-Lactate Dehydrogenase / metabolism Norepinephrine / metabolism* Rats Serotonin / metabolism Synaptosomes / enzymology* Tyrosine 3-Monooxygenase / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Immune Sera; 50-67-9/Serotonin; 51-41-2/Norepinephrine; 9007-36-7/Complement System Proteins; EC 1.1.1.27/L-Lactate Dehydrogenase; EC 1.14.16.2/Tyrosine 3-Monooxygenase; EC 1.14.17.1/Dopamine beta-Hydroxylase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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