| Specific inhibition of human telomerase activity by transfection reagent, FuGENE6-antisense phosphorothioate oligonucleotide complex in HeLa cells. | |
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MedLine Citation:
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PMID: 10431829 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Human telomerase might be associated with malignant tumor development and could be a highly selective target for antitumor drug design. Antisense phosphodiester (ODNs) and phosphorothioate (S-ODNs) oligonucleotides were investigated for their abilities to inhibit telomerase activity in the HeLa cell line. The ODNs and S-ODNs were designed to be complementary to nucleotides within the RNA active site of telomerase. As a transfection reagent, FuGENE6 was used to enhance the cellular uptake of oligonucleotides in cell cultures. The results showed that S-ODN-3 (19-mer) encapsulated with FuGENE6 clearly inhibited the telomerase activity in HeLa cells, and the inhibitory efficiency increased with an increase in the S-ODN-3. However, free S-ODN-3 showed no inhibitory activity. On the other hand, ODN-3 encapsulated with FuGENE6 had no detectable inhibitory activity. The encapsulated S-ODNs exhibited higher inhibitory activities than the free S-ODNs, and showed sequence specific inhibition. Thus, the activities of the S-ODNs were effectively enhanced by using the transfection reagent. The transfection reagent, FuGENE6, may thus be a potentially useful delivery vehicle for oligonucleotide-based therapeutics and transgenes, and is appropriate for use in vitro and in vivo. |
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Authors:
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M Tao; N Miyano-Kurosaki; K Takai; H Takaku |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: FEBS letters Volume: 454 ISSN: 0014-5793 ISO Abbreviation: FEBS Lett. Publication Date: 1999 Jul |
Date Detail:
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Created Date: 1999-08-26 Completed Date: 1999-08-26 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0155157 Medline TA: FEBS Lett Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 312-6 Citation Subset: IM |
Affiliation:
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Department of Industrial Chemistry, Chiba Institute of Technology, Narashino, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Enzyme Activation
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genetics Gene Expression Regulation, Enzymologic* Hela Cells Humans Indicators and Reagents Lipids / genetics* Oligonucleotides, Antisense / genetics* Telomerase / genetics*, metabolism Transfection* |
| Chemical | |
Reg. No./Substance:
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0/Indicators and Reagents; 0/Lipids; 0/Oligonucleotides, Antisense; EC 2.7.7.49/Telomerase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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