Document Detail


Specific inhibition of human telomerase activity by transfection reagent, FuGENE6-antisense phosphorothioate oligonucleotide complex in HeLa cells.
MedLine Citation:
PMID:  10431829     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Human telomerase might be associated with malignant tumor development and could be a highly selective target for antitumor drug design. Antisense phosphodiester (ODNs) and phosphorothioate (S-ODNs) oligonucleotides were investigated for their abilities to inhibit telomerase activity in the HeLa cell line. The ODNs and S-ODNs were designed to be complementary to nucleotides within the RNA active site of telomerase. As a transfection reagent, FuGENE6 was used to enhance the cellular uptake of oligonucleotides in cell cultures. The results showed that S-ODN-3 (19-mer) encapsulated with FuGENE6 clearly inhibited the telomerase activity in HeLa cells, and the inhibitory efficiency increased with an increase in the S-ODN-3. However, free S-ODN-3 showed no inhibitory activity. On the other hand, ODN-3 encapsulated with FuGENE6 had no detectable inhibitory activity. The encapsulated S-ODNs exhibited higher inhibitory activities than the free S-ODNs, and showed sequence specific inhibition. Thus, the activities of the S-ODNs were effectively enhanced by using the transfection reagent. The transfection reagent, FuGENE6, may thus be a potentially useful delivery vehicle for oligonucleotide-based therapeutics and transgenes, and is appropriate for use in vitro and in vivo.
Authors:
M Tao; N Miyano-Kurosaki; K Takai; H Takaku
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  FEBS letters     Volume:  454     ISSN:  0014-5793     ISO Abbreviation:  FEBS Lett.     Publication Date:  1999 Jul 
Date Detail:
Created Date:  1999-08-26     Completed Date:  1999-08-26     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0155157     Medline TA:  FEBS Lett     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  312-6     Citation Subset:  IM    
Affiliation:
Department of Industrial Chemistry, Chiba Institute of Technology, Narashino, Japan.
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MeSH Terms
Descriptor/Qualifier:
Enzyme Activation / genetics
Gene Expression Regulation, Enzymologic*
Hela Cells
Humans
Indicators and Reagents
Lipids / genetics*
Oligonucleotides, Antisense / genetics*
Telomerase / genetics*,  metabolism
Transfection*
Chemical
Reg. No./Substance:
0/Indicators and Reagents; 0/Lipids; 0/Oligonucleotides, Antisense; EC 2.7.7.49/Telomerase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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