Document Detail

Specific inhibition of the ABCG2 transporter could improve the efficacy of photodynamic therapy.
MedLine Citation:
PMID:  21911299     Owner:  NLM     Status:  Publisher    
Photodynamic therapy is based on the selective accumulation of a photosensitizer in tumors, followed by destruction of the target tissue by a light source. Protoporphyrin IX, a well-known photosensitizer, was recently reported as an endogenous substrate for the multidrug transporter ABCG2. We investigated the role of ABCG2 protein in the porphyrin extrusion ability of keratinocytes, with regard to the impact of the specific inhibition of ABCG2 by a non-toxic fumitremorgin C analog, Ko-134, on photodynamic therapy efficacy. We studied the level of porphyrin accumulation in response to delta-aminolevulinic acid pretreatment in proliferating and highly differentiated HaCaT keratinocytes. An in vitro model of photodynamic therapy on HaCaT cells was established with a therapeutically approved narrow-bandwidth red-light source. The porphyrin extrusion ability of HaCaT cells proved to correlate with their ABCG2 expression which was higher in proliferating cells than in differentiated cells. Moreover, the specific inhibition of ABCG2 by Ko-134 enhanced the sensitivity of keratinocytes to photodynamic therapy in vitro. These results suggest that ABCG2 may serve as a target molecule via which to improve the photodynamic therapy of skin lesions: its inhibition by the non-toxic Ko-134 is a promising therapeutic modality.
Attila Bebes; Tünde Nagy; Zsuzsanna Bata-Csörgő; Lajos Kemény; Attila Dobozy; Márta Széll
Related Documents :
22208719 - Dynamics of hbv cccdna expression and transcription in different cell growth phase.
21750649 - Microrna-7 inhibits the growth of human non-small cell lung cancer a549 cells through t...
22675209 - Regulated temporal-spatial astrocyte precursor cell proliferation involves braf signall...
21703549 - Deletion of the topoisomerase iii gene in the hyperthermophilic archaeon sulfolobus isl...
2761769 - Visual responses of sheep temporal cortex cells to moving and stationary human images.
21821009 - Persistent expression of nqo1 by p62-mediated nrf2 activation facilitates p53-dependent...
22819189 - Triamcinolone decreases bupivacaine toxicity to intervertebral disc cell in vitro.
9499459 - Progesterone stimulates production and secretion of muc1 epithelial mucin in steroid-re...
12452779 - Lack of cell death enhancement after irradiation with monochromatic synchrotron x rays ...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-8-23
Journal Detail:
Title:  Journal of photochemistry and photobiology. B, Biology     Volume:  -     ISSN:  1873-2682     ISO Abbreviation:  -     Publication Date:  2011 Aug 
Date Detail:
Created Date:  2011-9-13     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8804966     Medline TA:  J Photochem Photobiol B     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2011 Elsevier B.V. All rights reserved.
Department of Dermatology and Allergology, University of Szeged, Korányi fasor 6, Szeged H-6720, Hungary.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  An unusual co-presentation of rhinolithiasis and squamous cell carcinoma in the nasal cavity.
Next Document:  Clinical programs in the development of similar biotherapeutic products: Rationale and general princ...