| Specific inducibility of globin mRNA in Friend leukemia cells in low serum concentration. | |
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MedLine Citation:
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PMID: 7189734 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Friend leukemia cells were adapted to grow in a medium containing 0.5% serum by gradually decreasing concentration of the serum from 15%. Upon treatment of the cells with dimethyl sulfoxide, appearance of benzidine-positive cells was suppressed in the cells cultivated in a 0.5% serum medium, whereas globin mRNA sequences, as determined by hybridization of cytoplasmic RNA with globin cDNA, increased to the same order as in the cells grown in a medium containing 15% serum. A small but significant amount of globin was synthesized in cells treated with dimethyl sulfoxide in 0.5% serum medium, as determined by dodecyl sulfate-polyacrylamide gel electrophoresis of 3H-labeled cellular proteins. |
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Authors:
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K Nose; H Okamoto |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Gann = Gan Volume: 71 ISSN: 0016-450X ISO Abbreviation: Gann Publication Date: 1980 Feb |
Date Detail:
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Created Date: 1980-08-15 Completed Date: 1980-08-15 Revised Date: 2003-11-14 |
Medline Journal Info:
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Nlm Unique ID: 8214471 Medline TA: Gann Country: JAPAN |
Other Details:
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Languages: eng Pagination: 80-3 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Benzidines Blood* Cell Line Culture Media* Dimethyl Sulfoxide / pharmacology Friend murine leukemia virus Globins / biosynthesis* Hemoglobins / biosynthesis Leukemia, Experimental / metabolism* RNA, Messenger / biosynthesis* |
| Chemical | |
Reg. No./Substance:
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0/Benzidines; 0/Culture Media; 0/Hemoglobins; 0/RNA, Messenger; 67-68-5/Dimethyl Sulfoxide; 9004-22-2/Globins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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