Document Detail

Specific inducibility of globin mRNA in Friend leukemia cells in low serum concentration.
MedLine Citation:
PMID:  7189734     Owner:  NLM     Status:  MEDLINE    
Friend leukemia cells were adapted to grow in a medium containing 0.5% serum by gradually decreasing concentration of the serum from 15%. Upon treatment of the cells with dimethyl sulfoxide, appearance of benzidine-positive cells was suppressed in the cells cultivated in a 0.5% serum medium, whereas globin mRNA sequences, as determined by hybridization of cytoplasmic RNA with globin cDNA, increased to the same order as in the cells grown in a medium containing 15% serum. A small but significant amount of globin was synthesized in cells treated with dimethyl sulfoxide in 0.5% serum medium, as determined by dodecyl sulfate-polyacrylamide gel electrophoresis of 3H-labeled cellular proteins.
K Nose; H Okamoto
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Gan     Volume:  71     ISSN:  0016-450X     ISO Abbreviation:  Gan     Publication Date:  1980 Feb 
Date Detail:
Created Date:  1980-08-15     Completed Date:  1980-08-15     Revised Date:  2014-10-03    
Medline Journal Info:
Nlm Unique ID:  0151745     Medline TA:  Gan     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  80-3     Citation Subset:  IM    
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MeSH Terms
Cell Line
Culture Media*
Dimethyl Sulfoxide / pharmacology
Friend murine leukemia virus
Globins / biosynthesis*
Hemoglobins / biosynthesis
Leukemia, Experimental / metabolism*
RNA, Messenger / biosynthesis*
Reg. No./Substance:
0/Benzidines; 0/Culture Media; 0/Hemoglobins; 0/RNA, Messenger; 9004-22-2/Globins; YOW8V9698H/Dimethyl Sulfoxide

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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