Document Detail


Specific increase in calcium-activated neutral protease with low calcium sensitivity (m-calpain) in proerythroblastic K562 cell line cells induced to differentiation by phorbol 12-myristate 13-acetate.
MedLine Citation:
PMID:  1572412     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Changes in the level of calcium-activated neutral proteases (calpains) in K562 cells induced to differentiate by phorbol 12-myristate 13-acetate (PMA) were examined by an immunohistochemical technique and Western blot analysis. A remarkable increase in m-calpain (high-Ca(2+)-requiring form) level was detected after PMA-treatment, while there was no significant difference in mu-calpain (low-Ca(2+)-requiring form) level between PMA-treated and untreated K562 cells. To confirm whether the increase in m-calpain is specific to PMA-induced differentiation, we examined changes in calpain in K562 cells cultured in serum-free medium and in synchronized cells. The results indicate that the increase has no relation to growth arrest or to cell cycle. PMA-treated cells exhibited increased nonspecific esterase activity, suggesting monocytic differentiation. Immunoelectron microscopic study showed the reactions of dense deposits with monoclonal anti-m-calpain antibody on cell membranes, on membranes of coated vesicles, and on rough endoplasmic reticulum of K562 cells after 26 h of PMA treatment.
Authors:
M Nakamura; M Mori; Y Morishita; S Mori; S Kawashima
Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Experimental cell research     Volume:  200     ISSN:  0014-4827     ISO Abbreviation:  Exp. Cell Res.     Publication Date:  1992 Jun 
Date Detail:
Created Date:  1992-06-02     Completed Date:  1992-06-02     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0373226     Medline TA:  Exp Cell Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  513-22     Citation Subset:  IM    
Affiliation:
Department of Enzyme Biochemistry, Tokyo Metropolitan Institute of Gerontology, Japan.
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MeSH Terms
Descriptor/Qualifier:
Blotting, Western
Calpain / metabolism*
Cell Differentiation* / drug effects
Cell Nucleus / enzymology
Enzyme Activation / drug effects
Erythroblasts / enzymology*
Humans
Immunoenzyme Techniques
Leukemia, Myeloid, Chronic-Phase
Microscopy, Electron
Tetradecanoylphorbol Acetate / pharmacology*
Time Factors
Tumor Cells, Cultured
Chemical
Reg. No./Substance:
16561-29-8/Tetradecanoylphorbol Acetate; EC 3.4.22.-/Calpain

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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