| Specific increase in calcium-activated neutral protease with low calcium sensitivity (m-calpain) in proerythroblastic K562 cell line cells induced to differentiation by phorbol 12-myristate 13-acetate. | |
| | |
MedLine Citation:
|
PMID: 1572412 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Changes in the level of calcium-activated neutral proteases (calpains) in K562 cells induced to differentiate by phorbol 12-myristate 13-acetate (PMA) were examined by an immunohistochemical technique and Western blot analysis. A remarkable increase in m-calpain (high-Ca(2+)-requiring form) level was detected after PMA-treatment, while there was no significant difference in mu-calpain (low-Ca(2+)-requiring form) level between PMA-treated and untreated K562 cells. To confirm whether the increase in m-calpain is specific to PMA-induced differentiation, we examined changes in calpain in K562 cells cultured in serum-free medium and in synchronized cells. The results indicate that the increase has no relation to growth arrest or to cell cycle. PMA-treated cells exhibited increased nonspecific esterase activity, suggesting monocytic differentiation. Immunoelectron microscopic study showed the reactions of dense deposits with monoclonal anti-m-calpain antibody on cell membranes, on membranes of coated vesicles, and on rough endoplasmic reticulum of K562 cells after 26 h of PMA treatment. |
| | |
Authors:
|
M Nakamura; M Mori; Y Morishita; S Mori; S Kawashima |
Publication Detail:
|
Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Experimental cell research Volume: 200 ISSN: 0014-4827 ISO Abbreviation: Exp. Cell Res. Publication Date: 1992 Jun |
Date Detail:
|
Created Date: 1992-06-02 Completed Date: 1992-06-02 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 0373226 Medline TA: Exp Cell Res Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 513-22 Citation Subset: IM |
Affiliation:
|
Department of Enzyme Biochemistry, Tokyo Metropolitan Institute of Gerontology, Japan. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Blotting, Western Calpain / metabolism* Cell Differentiation* / drug effects Cell Nucleus / enzymology Enzyme Activation / drug effects Erythroblasts / enzymology* Humans Immunoenzyme Techniques Leukemia, Myeloid, Chronic-Phase Microscopy, Electron Tetradecanoylphorbol Acetate / pharmacology* Time Factors Tumor Cells, Cultured |
| Chemical | |
Reg. No./Substance:
|
16561-29-8/Tetradecanoylphorbol Acetate; EC 3.4.22.-/Calpain |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Transcriptional activation of the NCAM gene during myogenic differentiation.
Next Document: Induction of Calbindin-D28K by 1,25-dihydroxyvitamin D3 in cultured chicken intestinal cells.