| Specific immuno capturing of the staphylococcal superantigen toxic-shock syndrome toxin-1 in plasma. | |
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MedLine Citation:
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PMID: 19475676 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Toxic-shock syndrome is primarily caused by the Toxic-shock syndrome toxin 1 (TSST-1), which is secreted by the Gram-positive bacterium Staphylococcus aureus. The toxin belongs to a family of superantigens (SAgs) which exhibit several shared biological properties, including the induction of massive cytokine release and V(beta)-specific T-cell proliferation. In this study we explored the possibility to use monoclonal Variable domains of Llama Heavy-chain antibodies (VHH) in the immuno capturing of TSST-1 from plasma. Data is presented that the selected VHHs are highly specific for TSST-1 and can be efficiently produced in large amounts in yeast. In view of affinity chromatography, the VHHs are easily coupled to beads, and are able to deplete TSST-1 from plasma at very low, for example, pathologically relevant, concentrations. When spiked with 4 ng/mL TSST-1 more than 96% of TSST-1 was depleted from pig plasma. These data pave the way to further explore application of high-affinity columns in the specific immuno depletion of SAgs in experimental sepsis models and in sepsis in humans. |
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Authors:
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Hendrik Adams; Walter Brummelhuis; Bram Maassen; Nathalie van Egmond; Mohamed El Khattabi; Frank Detmers; Pim Hermans; Branko Braam; Jord Stam; Theo Verrips |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biotechnology and bioengineering Volume: 104 ISSN: 1097-0290 ISO Abbreviation: Biotechnol. Bioeng. Publication Date: 2009 Sep |
Date Detail:
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Created Date: 2009-07-27 Completed Date: 2009-09-21 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7502021 Medline TA: Biotechnol Bioeng Country: United States |
Other Details:
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Languages: eng Pagination: 143-51 Citation Subset: IM |
Copyright Information:
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(c) 2009 Wiley Periodicals, Inc. |
Affiliation:
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Faculty of Science, Department of Cellular Architecture and Dynamics, Utrecht University, Utrecht, The Netherlands. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antibodies, Monoclonal / immunology Antigens, Bacterial / immunology, isolation & purification* Bacterial Toxins / immunology, isolation & purification* Camelids, New World Chromatography, Affinity / methods* Enterotoxins / immunology, isolation & purification*, secretion Humans Plasma / chemistry* Protein Binding Sensitivity and Specificity Staphylococcus aureus / pathogenicity* Superantigens / immunology, isolation & purification* |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Monoclonal; 0/Antigens, Bacterial; 0/Bacterial Toxins; 0/Enterotoxins; 0/Superantigens; 0/enterotoxin F, Staphylococcal |
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