Document Detail


Specific glycine--accumulating synaptosomes in the spinal cord of rats.
MedLine Citation:
PMID:  4508336     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Subcellular fractionation of rat spinal cord on continuous sucrose density gradients provides evidence for the existence of a specific synaptosomal fraction (enriched in pinched-off nerve endings) that accumulates glycine selectively by way of a high-affinity transport system. The particles in this fraction sediment to a less-dense portion of sucrose gradients than do particles that accumulate neutral, basic, aromatic, and acidic amino acids. Particles accumulating gamma-aminobutyric acid are even less-dense than those storing exogenous glycine. The glycine-specific synaptosomal fraction also exists in the brain stem but not in the cerebral cortex. These findings provide neurochemical support for the suggestion that glycine has a specialized synaptic function, perhaps as neurotransmitter, in mammalian spinal cord.
Authors:
A Arregui; W J Logan; J P Bennett; S H Snyder
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  69     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  1972 Nov 
Date Detail:
Created Date:  1973-01-29     Completed Date:  1973-01-29     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  3485-9     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Aminobutyric Acids / metabolism
Animals
Arginine / metabolism
Aspartic Acid / metabolism
Brain Stem / cytology,  metabolism
Carbon Isotopes
Cell Fractionation
Centrifugation, Density Gradient
Cerebral Cortex / cytology,  metabolism
Glutamates / metabolism
Glycine / metabolism*
Leucine / metabolism
Lysine / metabolism
Male
Nerve Endings / metabolism*
Proline / metabolism
Rats
Spinal Cord / cytology,  metabolism*
Subcellular Fractions / metabolism
Synaptic Vesicles / metabolism*
Synaptosomes / metabolism
Threonine / metabolism
Tritium
Tyrosine / metabolism
Chemical
Reg. No./Substance:
0/Aminobutyric Acids; 0/Carbon Isotopes; 0/Glutamates; 10028-17-8/Tritium; 147-85-3/Proline; 55520-40-6/Tyrosine; 56-40-6/Glycine; 56-84-8/Aspartic Acid; 56-87-1/Lysine; 61-90-5/Leucine; 72-19-5/Threonine; 74-79-3/Arginine
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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