| Specific expression of the cell cycle regulation proteins, GADD34 and PCNA, in the peri-infarct zone after focal cerebral ischaemia in the rat. | |
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MedLine Citation:
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PMID: 12099899 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Cell cycle proteins play key roles in cell survival or death under pathological conditions. Expression of growth arrest and DNA damage-inducible protein, GADD34 and proliferating cell nuclear antigen (PCNA) have been investigated in the core and peri-infarct zone at 2 and 24 h after middle cerebral artery occlusion (MCAO). At these times after MCAO, numerous GADD34-positive cells were present, particularly in the peri-infarct zone (e.g. 24 +/- 4 and 52 +/- 6 immunopositive cells/0.25 mm2 at 2 and 24 h, respectively, in cortex). PCNA-immunopositive cells were barely detectable in the peri-infarct zone at 2 h; however, numerous PCNA-immunopositive cells were present in this zone by 24 h (0.7 +/- 0.3 and 10.6 +/- 1.5 immunopositive cells/0.25 mm2, respectively) as well as in the adjacent cortex and in the contralateral cingulate cortex. Most GADD34-immunopositive cells coexpressed the neuronal marker Neu-N with a smaller number coexpressing the microglial marker, Mrf-1. Evidence of morphologically 'abnormal' and 'normal' GADD34 immunopositive neurons was found within the peri-infarct zone. The majority of PCNA immunopositive cells were Mrf-1 positive with a smaller number Neu-N positive. Double-labelling revealed colocalization of GADD34 and PCNA in some cells within the peri-infarct zone and in the ependymal cells lining the ventricles. The presence of GADD34 and PCNA in a key anatomical location pertinent to the evolving ischaemic lesion indicates that GADD34, either alone or in combination with PCNA, has the potential to influence cell survival in ischaemically compromised tissue. |
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Authors:
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H Imai; J Harland; J McCulloch; D I Graham; S M Brown; I M Macrae |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The European journal of neuroscience Volume: 15 ISSN: 0953-816X ISO Abbreviation: Eur. J. Neurosci. Publication Date: 2002 Jun |
Date Detail:
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Created Date: 2002-07-08 Completed Date: 2002-09-04 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8918110 Medline TA: Eur J Neurosci Country: France |
Other Details:
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Languages: eng Pagination: 1929-36 Citation Subset: IM |
Affiliation:
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Wellcome Surgical Institute, University of Glasgow, Glasgow, UK. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Brain Ischemia / metabolism*, pathology, physiopathology Cell Cycle Proteins / metabolism* Cell Death / physiology* Cell Nucleus / metabolism, pathology Cell Survival / physiology* Cerebral Cortex / metabolism, pathology, physiopathology Cerebral Infarction / metabolism*, pathology, physiopathology Cytoplasm / metabolism, pathology DNA-Binding Proteins / metabolism Glial Fibrillary Acidic Protein / metabolism Immunohistochemistry Infarction, Middle Cerebral Artery / metabolism, pathology, physiopathology Male Neostriatum / metabolism, pathology, physiopathology Nerve Degeneration / metabolism, pathology, physiopathology Nerve Tissue Proteins / metabolism Neurons / metabolism, pathology Proliferating Cell Nuclear Antigen / metabolism* Proteins / metabolism* Rats Rats, Sprague-Dawley |
| Chemical | |
Reg. No./Substance:
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0/Cell Cycle Proteins; 0/DNA-Binding Proteins; 0/Glial Fibrillary Acidic Protein; 0/Nerve Tissue Proteins; 0/Proliferating Cell Nuclear Antigen; 0/Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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