| Specific effect of 5-fluorouracil on alpha-fetoprotein gene expression during the in vitro mouse embryonic stem cell differentiation. | |
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MedLine Citation:
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PMID: 20448262 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Embryonic stem (ES) cells are considered an important alternative to develop in vitro screening methods for embryotoxicity. Mouse ES cells can be cultured as cell suspension aggregates termed "embryoid bodies" (EBs) in which cells start to differentiate. We have studied the expression of several genes in the presence of a wide range of concentrations of 5-fluorouracil (5-FU). This well-established embryotoxic compound completely inhibited cell viability at 200 nmol/L in monolayer cultures. At lower concentrations, 5-FU led to decrease in the expression of the alpha-fetoprotein gene, a marker of the visceral endoderm, in the EBs. However, the expression of several mesodermal gene markers was not significantly affected at these concentrations. These results suggest a high sensitivity of the visceral endoderm differentiation to 5-FU. Therefore, the quantification of the alpha-fetoprotein gene after exposure to potential embryotoxicants should be considered an additional end point in future embryotoxicity assays in vitro with ES cells. |
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Authors:
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David Pamies; Néstor Vicente-Salar; Miguel A Sogorb; Enrique Roche; Juan A Reig |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: International journal of toxicology Volume: 29 ISSN: 1092-874X ISO Abbreviation: Int. J. Toxicol. Publication Date: 2010 May-Jun |
Date Detail:
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Created Date: 2010-05-07 Completed Date: 2010-08-27 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9708436 Medline TA: Int J Toxicol Country: United States |
Other Details:
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Languages: eng Pagination: 297-304 Citation Subset: IM |
Affiliation:
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Instituto de Bioingenieria, Universidad Miguel Hernández de Elche, Elche, Spain. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antigens, Differentiation / genetics, metabolism Cell Differentiation / drug effects* Cell Line Cell Survival / drug effects Dose-Response Relationship, Drug Embryonic Stem Cells / cytology, drug effects*, metabolism Endoderm / cytology, drug effects, metabolism Endpoint Determination Fluorouracil / toxicity* Gene Expression Regulation, Developmental / drug effects* Membrane Proteins / genetics, metabolism Mesoderm / cytology, drug effects, metabolism Mice Myosin Heavy Chains / genetics, metabolism Teratogens / toxicity* Time Factors Toxicity Tests / methods Vascular Endothelial Growth Factor Receptor-2 / genetics, metabolism alpha-Fetoproteins / genetics, metabolism* beta Catenin / genetics, metabolism |
| Chemical | |
Reg. No./Substance:
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0/Amn protein, mouse; 0/Antigens, Differentiation; 0/Catnb protein, mouse; 0/Membrane Proteins; 0/Myosin Heavy Chains; 0/Teratogens; 0/alpha-Fetoproteins; 0/beta Catenin; 0/beta myosin heavy chain protein, mouse; 51-21-8/Fluorouracil; EC 2.7.10.1/Vascular Endothelial Growth Factor Receptor-2 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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