Document Detail

Specific determination of endothelial cell viability in the whole cell fraction from cryopreserved canine femoral veins using flow cytometry.
MedLine Citation:
PMID:  11091174     Owner:  NLM     Status:  MEDLINE    
Abstract: An efficient method for specifically determining the viability of endothelial cells (EC) from cells dissociated from the human saphenous vein was investigated. Three different methods, trypan blue staining assay, [3H]-proline incorporation assay, and flow cytometry (FCM), combined with the fluorescein isothiocyanate conjugated with Griffonia simplicifolia agglutins (GS1-FITC)/propidium iodide (PI) double staining, were used. Both trypan blue staining and [3H] proline incorporation assays demonstrated less sensitivity to determine viability of EC differentially from the other cells. FITC-GS1 showed prominent binding to the vascular EC and could be counted by FCM including PI on dead cells. Following the cryopreservation process, the GS1-FITC/PI FCM analytical method was adopted to test simultaneously the viability of whole cells and EC from the same tissue, human saphenous veins, and mongrel dogs' femoral veins after harvesting, antibiotic solution treatment, and thawing. The viability of the whole cells from veins decreased with a significant difference (p < 0.05) from that of EC after thawing.
J C Park; H J Sung; D H Lee; Y H Park; B K Cho; H Suh
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Artificial organs     Volume:  24     ISSN:  0160-564X     ISO Abbreviation:  Artif Organs     Publication Date:  2000 Oct 
Date Detail:
Created Date:  2001-03-09     Completed Date:  2001-05-31     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  7802778     Medline TA:  Artif Organs     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  829-33     Citation Subset:  IM    
Department of Medical Engineering, Yonsei University College of Medicine, Seoul, Korea.
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MeSH Terms
Cell Survival
Endothelium, Vascular / cytology*,  metabolism
Femoral Vein
Flow Cytometry*
Middle Aged
Saphenous Vein
Staining and Labeling

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