Document Detail

Specific detection of tuberculosis infection: an interferon-gamma-based assay using new antigens.
MedLine Citation:
PMID:  15059788     Owner:  NLM     Status:  MEDLINE    
The tuberculin skin test for immunologic diagnosis of Mycobacterium tuberculosis infection has many limitations, including being confounded by bacillus Calmette-Guérin (BCG) vaccination or exposure to nontuberculous mycobacteria. M. tuberculosis-specific antigens that are absent from BCG and most nontuberculous mycobacteria have been identified. We examined the use of two of these antigens, CFP-10 and ESAT-6, in a whole blood IFN-gamma assay as a diagnostic test for tuberculosis in BCG-vaccinated individuals. Because of the lack of an accurate standard with which to compare new tests for M. tuberculosis infection, specificity of the whole blood IFN-gamma assay was estimated on the basis of data from people with no identified risk for M. tuberculosis exposure (216 BCG-vaccinated Japanese adults) and sensitivity was estimated on the basis of data from 118 patients with culture-confirmed M. tuberculosis infection who had received less than 1 week of treatment. Using a combination of CFP-10 and ESAT-6 responses, the specificity of the test for the low-risk group was 98.1% and the sensitivity for patients with M. tuberculosis infection was 89.0%. The results demonstrate that the whole blood IFN-gamma assay using CFP-10 and ESAT-6 was highly specific and sensitive for M. tuberculosis infection and was unaffected by BCG vaccination status.
Toru Mori; Mitsunori Sakatani; Fumio Yamagishi; Tetsuya Takashima; Yoshiko Kawabe; Keiji Nagao; Eriko Shigeto; Nobuyuki Harada; Satoshi Mitarai; Masaji Okada; Katsuhiro Suzuki; Yoshikazu Inoue; Kazunari Tsuyuguchi; Yuka Sasaki; Gerald H Mazurek; Izuo Tsuyuguchi
Related Documents :
20888298 - Optimization of inhaled therapies for tuberculosis: the role of macrophages and dendrit...
18201368 - Evaluation of a new recombinant bcg which contains mycobacterial antigen ag85b-mpt64(19...
16377228 - Vaccination strategies to prevent tuberculosis in the new millennium: from bcg to new v...
12065468 - Dna immunization in a mouse model of latent tuberculosis: effect of dna vaccination on ...
9302758 - Perspectives on fiv vaccine development.
16805708 - Pertussis immunisation in adolescents and adults--bordetella pertussis epidemiology sho...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2004-04-01
Journal Detail:
Title:  American journal of respiratory and critical care medicine     Volume:  170     ISSN:  1073-449X     ISO Abbreviation:  Am. J. Respir. Crit. Care Med.     Publication Date:  2004 Jul 
Date Detail:
Created Date:  2004-06-28     Completed Date:  2004-08-05     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  9421642     Medline TA:  Am J Respir Crit Care Med     Country:  United States    
Other Details:
Languages:  eng     Pagination:  59-64     Citation Subset:  AIM; IM    
Research Institute of Tuberculosis, 3-1-24 Matsuyama, Kiyose, Tokyo 204-8533, Japan.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Antigens, Bacterial / immunology*
BCG Vaccine / immunology
Bacterial Proteins / immunology*
Cross Reactions
Enzyme-Linked Immunosorbent Assay
Immunoassay / methods*
Immunologic Memory
Interferon-gamma / metabolism
Mycobacterium tuberculosis / immunology*
Sensitivity and Specificity
T-Lymphocytes / immunology
Tuberculin Test
Tuberculosis / diagnosis*
Reg. No./Substance:
0/Antigens, Bacterial; 0/BCG Vaccine; 0/Bacterial Proteins; 0/CFP-10 protein, Mycobacterium tuberculosis; 0/ESAT-6 protein, Mycobacterium tuberculosis; 0/Epitopes; 82115-62-6/Interferon-gamma
Comment In:
Am J Respir Crit Care Med. 2004 Jul 1;170(1):97-8; author reply 98   [PMID:  15220126 ]
Am J Respir Crit Care Med. 2004 Jul 1;170(1):5-6   [PMID:  15220119 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Pulmonary neutrophil infiltration in murine sepsis: role of inducible nitric oxide synthase.
Next Document:  Maternal atopic disease modifies effects of prenatal risk factors on exhaled nitric oxide in infants...