Document Detail


Specific detection of potentially allergenic peach and apple in foods using polymerase chain reaction.
MedLine Citation:
PMID:  22313353     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Two PCR methods were developed for specific detection of the trnS-trnG intergenic spacer region of Prunus persica (peach) and the internal transcribed spacer region of Malus domestica (apple). The peach PCR amplified a target-size product from DNA of six Prunus persica cultivars including two nectarine and one flat peach cultivars, but not from those of 36 nontarget species including six Prunus and five other Rosaceae species. The apple PCR amplified a target-size product from DNAs of five Malus domestica cultivars, but not from those of 41 nontarget species including seven Maloideae and nine other Rosaceae species. Both methods detected the target DNA from strawberry jam and cookies spiked with peach and apple at a level equivalent to about 10 g total soluble proteins of peach or apple/g incurred food. These specificity and sensitivity were considered sufficient for detection of trace amounts of peach or apple contamination in processed foods.
Authors:
Satoshi Watanabe; Hiromu Taguchi; Yusuke Temmei; Takashi Hirao; Hiroshi Akiyama; Shinobu Sakai; Reiko Adachi; Atsuo Urisu; Reiko Teshima
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-2-7
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  -     ISSN:  1520-5118     ISO Abbreviation:  -     Publication Date:  2012 Feb 
Date Detail:
Created Date:  2012-2-8     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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